PEMURNIAN PENISILIN ASILASE DARI BAKTERI BAC-4 DENGAN METODE KROMATOGRAFI AFINITAS HDROFOBIK

• Main Author: Muis, Abdul
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/2794
• Institution: Institut Teknologi Bandung

ABSTRACT:</b><br> <br /> <br /> BAC-4 is one of the local gram positive bacteria that produce penicillin acylase extracelullar enzyme. <br /> In this research, cells of BAC-4 was propagated in the culture medium containing sucrose with the optimum time for cell harvesting 10 hours after fermentation was began. The centrifugation methode was used for enzyme isolation and then purified by ultrafiltration, dialysis and hydrophobic affinity chromatography.</p> The enzyme activity was determined by PDAB (P-dimethylaminobenzaldehide) and the protein content was determined by Lowry methode, while poly acrylamide gel electroforese was used for determining the homogenity of the enzyme. <br /> The spesific activity for the purified enzyme was 1.052 unit/mg. The optimum pH was pH 7.0; temperature 40°C and incubation time 15 minutes. The kinetic data was KM was 2.53 mg/ml, Vm was 0.176 mcl 6 APA/minutes and K= was 6.20 mg/ml.