The Use of Synbiotic Kappaphycus alvarezii, Spirullina sp., and Bacillus cereus on Survival, Growth, and Vibriosis Resistance of White Shrimp (Litopenaeus vannamei) Post-larvae Culture

• Main Author: NUR FARIHA NIM : 10414020, IKA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/27946
• Institution: Institut Teknologi Bandung

<p align="justify"> <br /> <br /> Aquaculture is a growing sector and contributes to 50% of the world's marine products, one of them is white shrimp (Litopenaeus vannamei). Vibriosis is diseases that affect shrimp growth and decrease yield on shrimp production caused by Vibrio sp.. This research was conducted to see the effect of feed supplementation by using prebiotic Kappaphycus alvarezii and Spirullina sp. and probiotic Bacillus cereus against survival rates, growth, and Vibriosis resistance in white shrimp post-larvae (Litopenaeus vannmei). This research was conducted through 3 stages: (1) sinbiotic production and supplementation on feed; (2) feed performance test by using feed supplementation containing 0.375% K. alvarezii, 0.125% Spirullina, and Bacillus cereus at various concentration o108, 109, 1010 CFU/kg; and (3) challenge test using 106 cells / mL Vibrio harveyi. Following ten days of feed performance test, the highest shrimp survival of 89.62 ± 0.05% was obtained in treatment group fed with synbiotic-supplemented feed containing K. alvarezii : Spirullina at ratio of 3:1 and B. cereus at 108 CFU/kg (p<0.05). Challenge test was then performed using three treatment groups: (1) synbiotic with B. cereus at 108 CFU/kg; (2) prebiotic without B. cereus; and (3) control using commercial feed. During the tests, measurements of physical parameters (temperature, dissolved oxygen, pH, and salinity), chemistry (nitrates, nitrites, ammonium) were maintained at tolerance levels for shrimp post-larvae culture. Following five days of challenges test, the highest shrimp survival of 79.49±0.06% was found in treatment group fed with synbiotic with B. cereus at 108 CFU/kg, followed by prebiotic and control treatment of 56.62±0.05% and 39.49±0.04%, respectively (p<0.05). From this research, it is known that sinbiotic supplementation in feed can increase the survival, growth and resistance of white shrimp (Litopenaeus vannmei) with best composition of 0.375% K. alvarezii, 0.125% Spirullina and B. cereus at 108 CFU/kg. <br /> <p align="justify">