Molecularly Imprinted Silica Gel As Solid Phase Extraction Sorbent For Rhodamine B Analysis In Food Samples
<p align="justify">Rhodamine B is a red-violet dye commonly used in textile industry and as fluorescent molecular tag. Despite its well-known potent of carcinogenity and toxicity towards human through ingestion, Rhodamine B is still widely used as food colorant, including in Indonesi...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/28039 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <p align="justify">Rhodamine B is a red-violet dye commonly used in textile industry and as fluorescent molecular tag. Despite its well-known potent of carcinogenity and toxicity towards human through ingestion, Rhodamine B is still widely used as food colorant, including in Indonesia. Visible-light spectroscopy is one of the main methods used to determine Rhodamine B content. But, this method is prone to measuring error due to the matrix effect and possibility of another existing dyes with similar photophysic properties to Rhodamine B inside the sample. To anticipate that, in this research we have done the synthesis and characterization of Rhodamine B-Imprinted Silica Gel (RIS), and also studied its binding performance of Rhodamine B in order to perform a selective analysis of Rhodamine B. RIS was synthesized by imprinting the surface of silica gel with APTES (3-aminopropyl triethoxysilane) based on Stober process in order to bring out -NH2 group on its surface. The imprinting result has been confirmed by FTIR and SEM analysis. The binding process of Rhodamine B was studied by batch method. Acetonitrile was chosen as loading solvent due to its allowance of highest binding capacity compared to methanol and water. The optimum binding time of RIS was determined as 30 minutes. The binding process of Rhodamine B by RIS is following Freundlich adsorption isotherm model. Selectivity of RIS to other red dye such as Erythrosine and Ponceau 4R are consecutively 1.33 and 3.11. Synthesized RIS promises great measurement accuracy in solid phase extraction method, seen from its % recovery value of ~87%. Analysis of Rhodamine B content inside shrimp paste with RIS-SPE gives the result of 88.73 mg/kg.<p align="justify"> |
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