THE POTENTIAL OF SULFATE-REDUCING BACTERIA FROM OIL RESERVOIR X, SOUTH SUMATERA

• Main Author: Nyoman Samantha Adipranata Karang - Nim: 10414037, I
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28086
• Institution: Institut Teknologi Bandung

<p align="justify">Indonesia’s Oil and Gas Industries are currently entering the phase of Microbial Enhanced Oil Recovery (MEOR). One of the problems in MEOR’s application is the increasing of sulfide concentration from growth of Sulfate-Reducing Bacteria (SRB) that caused corrosion. Most researches are hindered by difficulties in culturing SRB on a laboratory scale. So, the purpose of this research is to determine the potential growth condition for SRB based on inoculum size and carbon source effect to its cell growth and sulfate-reduction activity. SRB sample were taken from oil reservoir X, South Sumatera before H2S biochemical test and were then enriched in Defined Media Widdel anaerobically (N2-flushed) in 37°C. 10%, 15%, dan 20% (v/v) variation were used to deteremine SRB growth condition. Cell count were measured with direct count method, chemical parameter (SO42- and S2-), with spectrophotometry, and pH measurement using pH meter. Carbon source variation includes Sodium Lactate (L1) as a control, n-hexane (C6) as aliphatic hydrocarbon compound, toluene (C7), dan xylene (C8) as aromatic hydrocarbon compound were tested with the same parameter with addition of NH4+ measurement with spectrophotometry. Results shows that sulfate reduction were present in the enrichment media containing SRB. 10% (v/v) inoculum shows best growth according to the calculation of specific growth rate (&#956;), sulfate reduction rate (&#956;reds), and sulfide production rate (Qps) in the amount of 0.0244 h-1, 0.0018 g/Lh, and 0.0040 mg/Lh respectively. L1 shows best growth according to &#956;, &#956;reds, and Qps in the amount of 0.0208 h-1, 0.0045 g/Lh, and 0.0112 mg/Lh, respectively. C8 were potentially used by SRB with &#956;, &#956;reds, and Qps in the amount of 0.0142 h-1, 0.0035 g/Lh, and 0.0039 mg/Lh, respectively. This research concluded that SRB can grow and reduce sulfate sulfat remarkably in Defined Media Widdel 37°C Anaerobic (N2-flushed) with 10% (v/v) (106 sel/ml) inoculum and sodium lactate (L1) and xylena (C8) as carbon source.<p align="justify">