Phycoremediation of Anaerobic Digested Dairy Manure Wastewater Using Chlorella vulgaris Exposed to Different Light Intensities

• Main Author: Nur Istiqomah (Nim : 11214030), Izmi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28207
• Institution: Institut Teknologi Bandung

Phycoremediation is a utilization of algae for the removal of pollutant from wastewater, including anaerobic digested dairy manure wastewater (ADDMW) that characterized by its high content of nitrogen and phosphorus compound. Chlorella vulgaris has high protein content and good ability to absorb nutrient, therefore potential to be utilized. One of the key parameters influencing microalgae growth is light, which provides energy for photosynthesis. This study intends to determine the optimal light intensity for the growth of C. vulgaris in ADDMW and reduction of its ammonium and orthophosphate content. Different light intensity of 50, 100 μmol/m2s and sunlight was applied to the culture. In addition, culture that is inoculated with C. vulgaris was compared with the culture which was not inoculated. Protein of the biomass is extracted by hot-TCA method in the end of the experiment. At light intensity 100 μmol/m2s, the biomass productivity and protein content show the best result which is 0,149 ± 0,03 gL-1d-1 and 10,74 ± 0,63% respectively. As for the specific growth rate and doubling time, the best result come from the culture which is cultured at sunlight that is 0,343 ± 0,034 d-1 and 2,028 ± 0,141 d respectively. Reduction rate of ammonium and orthophosphate are 36-48 mgL-1h-1 and 5-6,62 mgL-1h-1 respectively in culture which is inoculated with C. vulgaris. To conclude, C. vulgaris can act as a phycoremediator for ammonium and orthophosphate, therefore ADDMW can fulfill C. vulgaris nutrients’ needs with the appropriate environment, which light intensity is one of the parameters.