#TITLE_ALTERNATIVE#

• Main Author: TANUBRATA NIM : 10714096, JESSLYN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28275
• Institution: Institut Teknologi Bandung

Food and Agriculture Organization of the United Nations (FAO) has stated that one of the plants <br /> <br /> <br /> <br /> <br /> used as herbal medicine was bastard cedar (Guazuma ulmifolia Lamk.). In Indonesia bastard cedar <br /> <br /> <br /> <br /> <br /> plant is traditionally used as slimming agent, diarrhea drug, flatulence, abdominal pain and <br /> <br /> <br /> <br /> <br /> persistent cough. Bastard cedar can also be used to treat cancer. The compound which will play a <br /> <br /> <br /> <br /> <br /> big role in treating cancer is flavonoid compound. One of the flavonoid compounds in bastard <br /> <br /> <br /> <br /> <br /> cedar (Guazuma ulmifolia Lamk.) is tiliroside which is a flavonoid esther glycoside compound. <br /> <br /> <br /> <br /> <br /> Isolation of flavonoid compounds from bastard cedar has rarely been done. Flavonoid compound <br /> <br /> <br /> <br /> <br /> from bastard cedar (Guazuma ulmifolia Lamk.) showed anticancer effect. This study aim to isolate <br /> <br /> <br /> <br /> <br /> one of the flavonoid compounds from bastard cedar leaves which has a potential in treating <br /> <br /> <br /> <br /> <br /> cancer. Crude drug of bastard cedar leaves was extracted using reflux using ethanol 96% and <br /> <br /> <br /> <br /> <br /> monitored by thin layer chromatography (TLC) using flavonoid specific spray reagent which are <br /> <br /> <br /> <br /> <br /> AlCl3 and citroborate. The thick ethanol extract was fractionated by liquid-liquid extraction (LLE) <br /> <br /> <br /> <br /> <br /> method using n-hexane and ethyl acetate solvents, yielded n-hexane, ethyl acetate, and water <br /> <br /> <br /> <br /> <br /> fractions and monitored by TLC using citroborate spray reagent. In ethyl acetate fraction at least <br /> <br /> <br /> <br /> <br /> has four flavonoid spots and water fraction has one flavonoid spot, but in n-hexane fraction hasn’t <br /> <br /> <br /> <br /> <br /> a flavonoid spot. The ethyl acetate fraction was subfractionated by centrifugal thin layer <br /> <br /> <br /> <br /> <br /> chromatography (Chromatotron) and subfraction monitored by TLC using citroborate spray <br /> <br /> <br /> <br /> <br /> reagent. Subfractions was purified by preparative TLC, the isolate was tested for its purity by TLC <br /> <br /> <br /> <br /> <br /> using three different mobile phase system. The isolate was characterized by <br /> <br /> <br /> <br /> <br /> spectrophotodensitometry and two-dimensional paper chromatography. The isolate was <br /> <br /> <br /> <br /> <br /> predicted to be flavonol glycoside compund, with substituted OH group by sugar in C3 and free OH <br /> <br /> <br /> <br /> <br /> group in C5. <br />