A MULTI-OMICS APPROACH TO INVESTIGATE THE EFFECT OF CHITOSAN COATING ON POST-HARVEST QUALITY OF BANANA (MUSA ACUMINATA)

• Main Author: YAMAMOTO, KANA
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/28377
• Institution: Institut Teknologi Bandung

Bananaanana ananaanana is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit is a climacteric fruit whose post whose postwhose post -harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is harvest maturing process is affected by affected by affected by affected by affected by affected by affected by affected by respiration and ethylene production respiration and ethylene production respiration and ethylene production respiration and ethylene production respiration and ethylene productionrespiration and ethylene production respiration and ethylene productionrespiration and ethylene production respiration and ethylene productionrespiration and ethylene productionrespiration and ethylene productionrespiration and ethylene production respiration and ethylene productionrespiration and ethylene production respiration and ethylene production respiration and ethylene production respiration and ethylene production . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but . It is the most produced fruit in Indonesia but its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because its export is still low because the existing postexisting postexisting post existing post existing postexisting post -harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. harvest technologies are expensive. Chitosan coating is one of the costChitosan coating is one of the cost Chitosan coating is one of the cost Chitosan coating is one of the costChitosan coating is one of the cost Chitosan coating is one of the cost Chitosan coating is one of the cost Chitosan coating is one of the costChitosan coating is one of the cost Chitosan coating is one of the cost Chitosan coating is one of the costChitosan coating is one of the cost Chitosan coating is one of the costChitosan coating is one of the cost Chitosan coating is one of the costChitosan coating is one of the cost -effective posteffective posteffective posteffective posteffective posteffective post effective post effective posteffective post -harvest methods harvest methods harvest methods harvest methods that ca that cathat can extend extend extend extend extend fruit shelf fruit shelf fruit shelffruit shelf -life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan life. However, there are only a few studies on the effect of chitosan coating coating coating coating at the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the transcriptome and metabolome level. In this study, the the changes in changes in changes in changes in changes in changes in changes in changes in global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated global transcriptome and metabolome during the course of ripening uncoated and chand ch and chand chitosan itosan -coated banana were evaluatedcoated banana were evaluated coated banana were evaluatedcoated banana were evaluatedcoated banana were evaluated coated banana were evaluated coated banana were evaluated coated banana were evaluated coated banana were evaluatedcoated banana were evaluated coated banana were evaluated coated banana were evaluatedcoated banana were evaluatedcoated banana were evaluated coated banana were evaluated coated banana were evaluatedcoated banana were evaluated in order in order in order in order to obtain a better a better a better a better a better a better understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan understanding on the mechanisms involved in ripening delay upon chitosan treatment. treatment. treatment. treatment. treatment. Subsequently, the eSubsequently, the e Subsequently, the e Subsequently, the e Subsequently, the eSubsequently, the e Subsequently, the e Subsequently, the expression level of 1xpression level of 1 xpression level of 1xpression level of 1 xpression level of 1 xpression level of 1 xpression level of 1 xpression level of 1 xpression level of 1xpression level of 1-aminocyclopropanaminocyclopropan aminocyclopropan aminocyclopropanaminocyclopropanaminocyclopropan aminocyclopropan-1-carboxylic carboxylic carboxylic carboxylic carboxylic carboxylic carboxylic carboxylic acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme acid oxidase (ACO: ACC oxidase) genes, which encode an enzyme that convertconvert convert s ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. ACC to ethylene was also analyzed. The m The mThe metabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that etabolome data suggested that the the delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be r delay of banana ripening by chitosan coating might be rdelay of banana ripening by chitosan coating might be related to ethylene elated to ethylene elated to ethylene elated to ethylene elated to ethylene elated to ethylene elated to ethylene elated to ethylene elated to ethylene synthesis pathway. synthesis pathway. synthesis pathway. synthesis pathway. synthesis pathway. synthesis pathway. synthesis pathway. synthesis pathway. synthesis pathway. .Furthermore, Furthermore, Furthermore, Furthermore, Furthermore, Furthermore, Furthermore, Furthermore, according to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some ccording to the transcriptome analysis, some interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were interesting genes were shown based on log based on log based on log based on log based on log 2-fold change value fold change valuefold change value fold change valuefold change valuefold change valuefold change valuefold change value fold change value and it and it and it and it and it also also also also suggested that suggested that suggested that suggested that chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway chitosan coating might affect the ethylene synthesis pathwaychitosan coating might affect the ethylene synthesis pathway. qPCR . qPCR . qPCR . qPCR res result showed ult showed ult showed ult showed two ACO genes (ID: Ma05_t09360.1, Ma10_t01130.1) had higher expression levels in chitosan-coated banana. It was considered that chitosan coating might inhibit the ethylene production in the step of ACC to ethylene by adjusting the permeability of surrounded O2 and inhibiting the reaction of ACO. This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and This is the first study to report difference between untreated and chitosanchitosan chitosan -coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level. coated banana in transcriptome and metabolome level.coated banana in transcriptome and metabolome level. coated-