#TITLE_ALTERNATIVE#

• Main Author: AULIA RAHMAH NIM : 10713014, KHAIRUNNISSA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28464
• Institution: Institut Teknologi Bandung

Vaccination is the administration of immunogen to induce an active immunity. Oral vaccination is <br /> <br /> not invasive but has lower bioavailability than parenteral vaccinations. The study aimed to develop <br /> <br /> an oral vaccine preparation containing Bovine Serum Albumin (BSA) and Moringa leaf water <br /> <br /> extracts Moringa oleifera Lamk. (MO) nanoparticle coated with sodium alginate, Eudragit S100, and <br /> <br /> Eudragit FS 30D. Nanoparticle was prepared by ionotropic gelation method using chitosan polymer <br /> <br /> as matrix and sodium tripolyphosphate as cross-linker. The particle size and polydispersity index <br /> <br /> were characterized using the DelsaTM NanoC (Beckman Coulter) instrument, the entrapment <br /> <br /> efficiency was determined using the Bradford method (for BSA) and the Chang method (for extract), <br /> <br /> the release profile was evaluated in vitro, and the immune response was evaluated in vivo in BALB/c <br /> <br /> strain mice by determining antibody titer using the immunodifusion method. The results of in vitro <br /> <br /> showed Eudragit S100 is the best polymer in preventing BSA release in gastric pH (1,2) and the <br /> <br /> fastest polymer in releasing BSA at pH ileum (7,4). The result of in vivo showed BSA-MO (100 &#956;g/mL) <br /> <br /> coated with sodium alginate induce the highest humoral immune response base on antibody titer <br /> <br /> (1:128) compared to other orally administered nanoparticle although still lower than parenteral <br /> <br /> administration (1:256). From these results obtained that oral vaccine preparation containing BSA <br /> <br /> and Moringa leaf water extract nanoparticle coated with sodium alginate capable of inducing a <br /> <br /> highest humoral immune response compared with other nanoparticle preparations although <br /> <br /> immunogenicity is lower than parenteral administration. <br />