#TITLE_ALTERNATIVE#

• Main Author: NURUL SAKINAH NIM : 11614011, LIA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28591
• Institution: Institut Teknologi Bandung

Dyslipidemia is a major risk factor for various cardiovascular diseases such as atherosclerosis, <br /> <br /> coronary heart disease, myocardiac infarction, and hypertension. Various studies have shown that <br /> <br /> pomegranate juice and pomegranate peels have various activities such as antiobesity, antidiabetic, <br /> <br /> and antihyperlipidemia. The availability of pomegranate leaves that more than pomegranate fruits <br /> <br /> makes pomegranate leaves a natural ingredient that is very potential for further investigation. The <br /> <br /> purpose of this study was to determine the antidislipidemia activity of pomegranate leaf ethanol <br /> <br /> extract on male Wistar rats. The extract was obtained by reflux method with 96% ethanol. The study <br /> <br /> used 25 male Wistar rats which were divided into 5 groups namely, negative control group, positive <br /> <br /> control group, simvastatin comparison group 10 mg/kg bw, extract group 100 and 300 mg/kg bw. <br /> <br /> The method of induction of dyslipidemia used was induction by giving high-fat foods and pure <br /> <br /> cholesterol of 200 mg/kg bw, propylthiouracil (PTU) 12.5 mg/kg bw, and 0.1% colic acid for 12 <br /> <br /> weeks. After the dyslipidemia model was formed, pomegranate leaf extract and simvastatin were <br /> <br /> administered orally to test animals. The main parameters measured were total cholesterol, <br /> <br /> triglycerides, HDL with enzymatic methods, LDL with calculations using Friedewald formula, <br /> <br /> atherogenic index, and histology test. These parameters were measured before induction, after <br /> <br /> induction, after 2 weeks of treatment, and 1 month of treatment. At the end of the experiment, all <br /> <br /> animals were sacrificed. The heart and aorta are isolated. The results showed that induction of highfat foods for 12 weeks could increase total cholesterol levels in groups of dyslipidemic animals <br /> <br /> significantly compared to negative groups. After 2 weeks of therapy, simvastatin at a dose of 10 <br /> <br /> mg/kg bw reduced total cholesterol levels by 43.93%; reduced LDL levels by 85.75%; and increased <br /> <br /> HDL levels by 99.72%. Pomegranate leaf ethanol extract with doses of 100 and 300 mg/kg bw <br /> <br /> decreased total cholesterol levels by 27.6% and 60.11%, respectively. In addition, ethanol extract <br /> <br /> of pomegranate leaves with doses of 100 and 300 mg/kg bw also decreased LDL levels by 34.79% <br /> <br /> and 78.05%, respectively. After 1 month of therapy, simvastatin at a dose of 10 mg/kg bw reduced <br /> <br /> total cholesterol levels by 57.1%; reduced LDL levels by 84.48%; and increased HDL levels by <br /> <br /> 125.17%. Pomegranate leaf ethanol extract with a dose of 100 mg/kg bw decreased total <br /> <br /> cholesterol levels by 59.88% and decreased LDL levels by 75.05%. Pomegranate leaf ethanol extract <br /> <br /> also reduced the atherogenic index and the thickness of the aortic wall. From these results, it can <br /> <br /> be concluded that the ethanol extract of pomegranate leaves has potential as antidyslipidemia. <br />