#TITLE_ALTERNATIVE#

• Main Author: AFRIYANI NIM : 10714063 , LINA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28604
• Institution: Institut Teknologi Bandung

Propionibacterium acne and Staphylococcus epidermidis are the major bacteria that infect skin which can <br /> <br /> cause many disease in the skin, one of them is acne. Papaya is one of the plants that has many activities, such <br /> <br /> as antibacterial activity. In traditional use, papaya leaves used for treatment of acne. In this study, the <br /> <br /> antibacterial activity of n-hexane, ethyl acetate, and ethanol papaya leaves extract were tested against <br /> <br /> Propionibacterium acne and Staphylococcus epidermidis ATCC 12228 using agar diffusion disc method, <br /> <br /> microdilution method to determine minimum inhibitory concentration (MIC), and bioautography test. The <br /> <br /> preliminary result with diffusion disc showed that at 5% concentration of n-hexane extract of papaya leaves <br /> <br /> produced the largest inhibit zone for both bacteria, 7,43 mm for Propionibacterium acne and 7,78 mm for <br /> <br /> Staphylococcus epidermidis. The result showed that the MIC of n-hexane extract of papaya leaf were >4096 <br /> <br /> µg/mL. The result of the bioautography test of n-hexane extract showed the inhibition zone at Rf 0,09-0,28 <br /> <br /> and Rf 0,63 in chloroform-n-hexane system (6:3) for Propionibacterium acne. Based on the test result, the nhexane extract of papaya leaves was continued to the fractination stage by chromatotron method and the <br /> <br /> compound at Rf 0,27 were selected as the target. The fraction was monitored by thin-layer chromatography <br /> <br /> (TLC) and the fraction contained the active compound was continued to subfractination stage by preparative <br /> <br /> chromatography method. The subfraction was tested against Propionibacterium acne and Staphylococcus <br /> <br /> epidermidis by bioautography method at 600 µg/mL concentration. It resulted an inhibitory zone at Rf 0,25- <br /> <br /> 0,27 for Staphylococcus epidermidis and Rf 0,27 for Propionibacterium acne. Based on the result <br /> <br /> characterization of subfraction of n-hexane extract of papaya leaves by specific visualization reagent, the <br /> <br /> subfraction was predicted contain steroid/triterpenoid compound. <br /> <br />