#TITLE_ALTERNATIVE#
Indonesia is a country where infection is still a common disease. One of the bacteria that can <br /> <br /> cause infection and a common one is Escherichia coli. This bacteria can be treated by beta-lactam <br /> <br /> antibiotics. Third generation of sephalosporin, ceftria...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/28730 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Indonesia is a country where infection is still a common disease. One of the bacteria that can <br />
<br />
cause infection and a common one is Escherichia coli. This bacteria can be treated by beta-lactam <br />
<br />
antibiotics. Third generation of sephalosporin, ceftriaxone, is antibiotic that included in the <br />
<br />
guideline for E. coli infection treatment. Excessive use and misuse of antibiotics can lead to <br />
<br />
bacteria resistance of certain antibiotics. The mechanism can be making beta-lactamase enzymes. <br />
<br />
The purpose of this research is to determine the subgenotipe of blaCTX-M gene from the clinical <br />
<br />
isolate of E. coli that resistance to ceftriaxone and get treatment recommendation for patient that <br />
<br />
infected by E. coli that resistance to ceftriaxone based to the result of this research. The blaCTX-M <br />
<br />
gene in clinical isolates of E. coli that resistance to ceftriaxone was checked by Polymerase Chain <br />
<br />
Reaction (PCR) with the colony as DNA template. The PCR result then visualized by agarose <br />
<br />
electrophoresis to see the fluoroscence on the electrophoregram that show the blaCTX-M gene. <br />
<br />
Samples that contain blaCTX-M gene was sequenced. The sequences were aligned to blaCTX-M gene <br />
<br />
in software GenBank and homologous/identity persentages were determined to get the <br />
<br />
subgenotipe of blaCTX-M gene. From 15 samples that have blaCTX-M gene, 5 of the sample has blaCTX- <br />
<br />
M-15 and was chosen to be sequenced. All of them contained blaCTX-M-15 gene with 100% <br />
<br />
homologous. E. coli that contain blaCTX-M-15 resistant to various beta-lactam antibiotics. Antibiotics <br />
<br />
recommendation for treat E. coli that contain blaCTX-M-15 is carbapenem subgroup and betalactams with tazobactam combination. <br />
|
|---|