PRODUCTION OF ENZYMATIC MICROCELLULOSE FROM KOMBUCHA SCOBY WITH CRUDE EXTRACT OF CELLULASE ENZYME FROM Bacillus cereus

• Main Author: Fransisca Suwendi - Nim: 10414032, Maria
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28747
• Institution: Institut Teknologi Bandung

<p align="justify">In kombucha fermentation, in addition to the main products in the form of beneficial, fresh and low alcoholic beverages, side products such as kombucha membranes or SCOBY are also produced and composed of cellulose. The SCOBY was then used as raw material for cellulose in this study. Microcellulose is commonly used in the pharmaceutical industry as fillers and binders in capsule making, in the food industry as emulsifiers and compacting agents, in the cosmetic industry as emulsifiers, absorbers, and refiners of cosmetic ingredients. The demand for microcellulose as a biopolymer in various industries, which is increasingly high, causes microcellulose production to be carried out effectively and efficiently. Although it can be made physically and chemically, the enzymatic manufacture of microcellulose is environmentally friendly, and more efficient in term of energy, so it is good to do. Based on that, this study aims to produce microcellulose from SCOBY kombucha enzymatically through the help of Bacillus cereus organisms with variables in the form of enzyme activity and incubation time. The method used consists of cellulase enzyme production and microcellulose manufacturing. Crude extract of cellulase was obtained from Bacillus cereus culture grown in CMC medium for up to 60 hours at 37°C and agitated at 150 rpm, and then the cell and medium were separated. Before enzyme addition, the cellulose substrate in the form of SCOBY are dried first and crushed to a powder. In the process of making microcellulose, SCOBY powder was suspended in citrate buffer and given 2, 8 and 20 units/mL of crude extract of cellulase from Bacillus cereus. After that, it was incubated at 45°C and agitated at 150 rpm for 3 and 5 days. The highest percentage of cellulose particles with a size range of 0.1-1 &#956;m was obtained at 87.94% on the treatment of 2 U/mL enzyme and incubated for 5 days.<p align="justify">