#TITLE_ALTERNATIVE#

• Main Author: ASMARADITA DAHTIAR NIM : 10714050, MARSHA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28771
• Institution: Institut Teknologi Bandung

Chlorphenesin and phenoxyethanol are preservatives that commonly used in cosmetics. These <br /> <br /> two preservatives are often combined to make cosmetic products more resistant to microbes <br /> <br /> with a boarder spectrum. Chlorphenesin and phenoxyethanol are relatively safe preservatives if <br /> <br /> the amount added to the preparation does not exceed the maximum permissible limit of use, <br /> <br /> 0.3% for chlorphenesine and 1% for phenoxyethanol. Therefore, controlling the content of <br /> <br /> chlorphenesin and phenoxyethanol in cosmetics is important. This research has developed a <br /> <br /> simultaneous analysis for the content of these two compounds in the compact powder sample <br /> <br /> using the HPLC instrument. The HPLC system used included 4.6 x 150 mm Inertsil ODS-3 column as <br /> <br /> a stationary phase, phosphate buffer pH 3.5 and acetonitrile mixture at 70:30 as a mobile phase, <br /> <br /> UV-Vis spectrophotometer detector at 279 nm detection wavelength, 1 mL / minute flow rate and <br /> <br /> 10 &#956;L injection volume. The method used can analyze the substance chlorphenesin and <br /> <br /> phenoxyethanol with resolution > 1,5. This method also provides good linearity for chlorphenesin <br /> <br /> in the range 6-21 &#956;g / mL and 20-70 &#956;g / mL for phenoxyethanol. Detection limits for <br /> <br /> chlorphenesin were 1.098 &#956;g / mL and 1.426 &#956;g / mL for phenoxyethanol. The recovery for <br /> <br /> chlorphenesin was 84.957 - 108.425% and the recovery of phenoxyethanol was 83.277 - <br /> <br /> 107.9249%. The method developed has fulfilled the validation requirements and can be applied to <br /> <br /> analyze the content of chlorphenesin and phenoxyethanol in six compact powder samples in the <br /> <br /> range 0.013-0.170% (w / w) and phenoxyethanol content in the range 0.052 - 0.167% (w / w). <br />