#TITLE_ALTERNATIVE#

• Main Author: ZAFITRI LUBIS NIM : 10714097, MAULIDA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/28804
• Institution: Institut Teknologi Bandung

Cinchona bark (Cinchona officinalis L., Rubiaceae) contains several alkaloid compounds which <br /> <br /> <br /> <br /> have been used as medicine. Meanwhile, research on the content of non-alkaloid compounds <br /> <br /> <br /> <br /> from cinchona bark is still limited, therefore, in this study flavonoid compounds were isolated <br /> <br /> <br /> <br /> from cinchona bark. A concentrated extract obtained from PT Sinkona Indonesia Lestari was <br /> <br /> <br /> <br /> monitored by thin layer chromatoghraphy (TLC). The alkaloid content in the extract was <br /> <br /> <br /> <br /> separated by the acid-base extraction and fractionated by liquid-liquid extraction using ethyl <br /> <br /> <br /> <br /> acetate. The alkaloid-free ethyl acetate fraction was monitored by TLC to determine the target <br /> <br /> <br /> <br /> compound. The ethyl acetate fraction was subfractionated by radial chromatography and <br /> <br /> <br /> <br /> monitored by TLC. The subfractions that contain the target compound were collected and <br /> <br /> <br /> <br /> purified by preparative TLC. The purification results were tested for purity by TLC with three <br /> <br /> <br /> <br /> single developing systems. The isolate was characterized using ultraviolet-visible <br /> <br /> <br /> <br /> spectrophotometry, ammonia vapor exposure, and two-dimensional paper chromatography. <br /> <br /> <br /> <br /> The isolate showed maximum absorption at 282 nm and 320 nm and was suspected as a <br /> <br /> <br /> <br /> flavanone aglycone having a free 7-hydroxyl group. <br />