In vitro Micropropagation of Moss Taxithelium kerianum (Broth.) Fleisch and Leucobryum aduncum Dozy & Molk

• Main Author: YUSUF FAHREZA - Nim: 10613069 , MOHAMAD
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/29005
• Institution: Institut Teknologi Bandung

<p align="justify">Mosses are the second largest terrestrial plants, yet the information of ethnobryology and the application of mosses as medicines are very limited. Therefore, this study was designed to optimize in vitro propagation of the selected mosses Taxithelium kerianum and Leucobryum aduncum. For this, both spore of T. kerianum and gametophyte explants of L. aduncum were surfaced sterilized by using fungicide, NaClO, cloroxylenol, ethanol and HgCl2. Subsequently, these explants were grown in either Murashige and Skoog (MS) or Knop medium supplemented with napthalene aceticacid (NAA), 3-indole aceticacid (IAA), kinetin, zeatin, and gibberelic acid (GA3). The results showed that cloroxylenol 0.48% (10 min) + ethanol 70% (5 sec) + HgCl2 0.025% (5 min) were effective to sterilize T. kerianum spores, while cloroxylenol 0.48% (5 min) + ethanol 70% (5 sec) + HgCl2 0.025% (5 min) were effective for L. aduncum gametophore sterilization. Furthermore, growth of both species were significantly better in Knop medium compared to basal MS medium. The addition of growth hormones also resulted in different responses on the development of protonema from T. kerianum. IAA induced the new formation of protonema filaments, kinetin induced gametophores branching, while GA3 induced stem elongation and shoot multiplication. In contrast, none of these hormone treatments influenced growth and development of L. aduncum gametophore. The obtained results will be critical not only to study the development of different moss spesies, but also to provide sufficient biomass of the selected mosses for various purposes.<p align="justify"> <br />