#TITLE_ALTERNATIVE#

• Main Author: LINA ROHMAWANTI , NENG
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/29603
• Institution: Institut Teknologi Bandung

<p align="justify">Bacteriophage is another alternative in using antibiotics to fight vibriosis in white shrimp (Litopenaeus vannamei). Vibriosis is a disease of shrimp that can kill shrimp mass (70%), thereby reducing shrimp production for farmers. Vibriosis is a disease caused by Vibrio sp. This aims of this study are to (1) isolate Vibrio sp. bacteria from water samples from white shrimp ponds in Situbondo & Bali, (2) isolate bacteriophages from water samples of white shrimp ponds in Situbondo & Bali, (3) determine the effectiveness of phage infection on inhibition of Vibrio sp. during enrichment and (4) determine comparison of the bacteriophage enriched isolation samples from Situbondo & Bali with a sample that cause vibriosis in white shrimp. Water samples that cause vibriosis in shrimp are used as positive control (IR code). The stages of this study are sequentially isolation of Vibrio spp. bacteria. with Thiosulfate-Citrate-Bile Salt-Sucrose Agar; making a standard curve & a growing curve with Luria Bertani (LB); bacteriophage isolation, bacteriophage enrichment and bacteriophage challenge test to Vibrio sp. respectively. Isolation results with the Double Layer Agar technique include plaque test, purification and determination of phage titer with ZnCl2 (aq). The results of bacterial isolation from the three places of Balai Badan Perikanan Budidaya Air Payau (BPBAP) Situbondo, Balai Benih Udang dan Ikan (BBUI) Situbondo, and UD Simpati Bali obtained 25 bacterial isolates including 8 as candidates for Vibrio sp., and only one as V. alginolyticus. Bacteriophages were isolated in the logarithmic phase of V. alginolyticus infection (1,5 hours) from water samples which caused vibriosis (IR code), BPBAP water sample (BP code) and stationary phase (5,5 hours) BPBAP water (BP2 code) successive enrichment results obtained 1,08 x 102 PFU / mL clear zone Ø0,5-1 mm; 2,0 x 10 PFU / mL and 1,0x10 PFU / mL clear zone with white in the center of Ø0,5-1 mm. Bacteriophages are more isolated in the logarithmic infection of V. alginolyticus and are most isolated in water samples that cause vibriosis.<p align="justify">