#TITLE_ALTERNATIVE#

• Main Author: MUHLISAH MADDEPPUNGENG NIM : 20716014, NURUL
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/29842
• Institution: Institut Teknologi Bandung

Background and purpose: The case of malaria is still a global problem and still faces <br /> <br /> many challenges. The use of antimalaria medicines has been reported to be a lot of failure <br /> <br /> against protozoa Plasmodium due to resistance. Lack of structural diversity of <br /> <br /> antimalarial compounds and the increasing resistance of parasite to the existing drugs <br /> <br /> drive the need to develop new & effective antimalarial agents. L-Malate Enzymes: <br /> <br /> Quinone Oxidoreductase (MQO) is a functional enzyme that works in the mitochondrial <br /> <br /> electron transport chain. MQO is a specific enzyme that exists only in Plasmodium <br /> <br /> parasite that will be targeted drugs. Endophytic fungi have been known to produce <br /> <br /> bioactive secondary metabolites. The objectives of this research were to isolate and <br /> <br /> purify secondary metabolite which inhibits the activity of Plasmodium falciparum Malate <br /> <br /> Quinone Oxideductase (PfMQO) enzyme. Methodes: extraction was done using <br /> <br /> maseration method with methanol yielded 2,1 g dry extract. Isolation was done by column <br /> <br /> chromatography with silica gel as adsorben then with column chromatography sephadex <br /> <br /> LH-20, and by preparative TLC. Structur elucidation was determined using maximum <br /> <br /> wave lenght and mass spectroscopy data. Result: Based on molecular identification <br /> <br /> showed that endophytic isolate fungi P3 was known as Corynespora torulosa. Pure <br /> <br /> compound showed the inhibition of PfMQO enzyme activity. Conclutions: Isolation and <br /> <br /> purification secondary metabolite of Corynespora torulosa known as purpurasolol with <br /> <br /> IC50 92,4 ug/ml agains PfMQO. <br />