#TITLE_ALTERNATIVE#

• Main Author: AYU ANDINI NIM : 10714100, PARAMITHA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/29912
• Institution: Institut Teknologi Bandung

Staphylococcus aureus (SA) has been known to causes intracellular and extracellular infections. <br /> <br /> Gentamicin sulfate is one of the drug of choice for SA infections, but it has low intracellular <br /> <br /> penetration ability. Nanostructured lipid carriers (NLC) and chitosan have been proposed to <br /> <br /> increase intracellular penetration of antibiotics. This study aims to develop formulas for gentamicin <br /> <br /> sulfate in NLC with and without chitosan modification. Gentamicin was emulsified in glyceryl <br /> <br /> monooleate (GMO) and oleic acid using mixture of tween 80 and span 80 as emulgator to make <br /> <br /> primary emulsion W1/O. The primary emulsion was further emulsified with high or low molecular <br /> <br /> weight chitosan (HMWC/LMWC) and tween 80 and propylene glycol as emulgator to form W/O/W <br /> <br /> multiple emulsion. Emulsions containing chitosan was added sodium tripoliphosphate (STPP) as a <br /> <br /> cross-linker. The three nanoparticles produced had high encapsulation efficiency of 89-98%, <br /> <br /> particle size in the range of 149-933 nm, and a uniform particle size distribution with narrow <br /> <br /> polydispersity index of 0.31-0.42. It is shown that the three nanoparticles had lower antimicrobial <br /> <br /> activity than gentamicin solution and had higher drug release profile at pH 7.4 compared to pH 5.2 <br /> <br /> for NLC without chitosan and NLC with LMWC. NLC with HMWC had burst release at pH 7.4 and <br /> <br /> had similar release profile as the other formulas at pH 5,2. The conclusion of this study is the NLC <br /> <br /> formula without chitosan and the NLC formula with LMWC have potential to be developed for <br /> <br /> intracellular targeting of gentamicin sulfate. <br />