#TITLE_ALTERNATIVE#

• Main Author: NURWIDAYANTI (NIM : 10414001), PURI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30003
• Institution: Institut Teknologi Bandung

<p align="justify"> Litopenaeus vannamei or white shrimp has become the primary species currently being cultured in South-East Asian countries. The occurrence of infectious diseases in shrimp aquaculture is a serious problem in the production process such as vibriosis that can cause economic losses. The research was aimed to determine the effect of feed supplementation by using synbiotic containing prebiotics red seaweed Kappaphyucs alvarezii, microalgae Spirullina sp. and probiotic Halomonas alkaliphila on survival, growth, and vibriosis resistance of white shrimp (Litopenaeus vannamei) post-larvae. The methods were: (1) synbiotic production and feed supplementation; (2) viability of probiotics test on synbiotic feed; (3) feed performance test by using feed supplementation containing 0.375% K. alvarezii, 0.125% Spirullina, and H. alkaliphila at various concentration of 108, 109, and 1010 CFU/kg; and (4) challenge test by using 106 CFU/ml Vibrio harveyi. Following ten days of feed performance test, the highest shrimp survival of 91.46±0.05% was obtained in treatment group fed with synbiotic-supplemented feed containing 0.375% K. alvarezii, 0.125% Spirullina and H. alkaliphila at 109 CFU/kg (p<0.05). Challenge test was then performed using three treatment groups: (1) synbiotic with H. alkaliphila at 109 CFU/kg; (2) prebiotic without H.alkaliphila; and (3) control using commercial feed. During the experiment, physicochemical water quality parameters (temperature, dissolved oxygen, pH, salinity, nitrate, nitrite, and ammonium concentration) were maintained at tolerance levels for shrimp post-larvae culture. Following five days of challenges test, the highest shrimp survival of 79.9±0.05% was found in treatment group fed with synbiotic with H. alkaliphila at 109 CFU/kg, followed by prebiotic and control treatment of 56.62±0.05% and 39.49±0.04%, respectively (p<0.05). Overall, it can be suggested that feed supplementation using synbiotic containing 0.375% K. alvarezii, 0.125% Spirullina and H. alkaliphila at 109 CFU/kg can significantly improve shrimp survival even after challenged with V. harveyi. This synbiotic feed supplementation can be potentially applied as one alternative of biocontrol strategy against vibriosis in shrimp post-larvae culture. <p align="justify">