Functional Feed from High Carotene Bananas (Musa troglodytarum) to Modulate Immune Response in Zebrafish Intestine (Danio rerio)
<p align="justify">Bananas are known as functional food, because they not only contain many nutrients that is needed by the body, but are also expected to improve health and can help to cure some diseases. One of banana species which is known to have a lot of nutrients is Tongka Lang...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/30043 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <p align="justify">Bananas are known as functional food, because they not only contain many nutrients that is needed by the body, but are also expected to improve health and can help to cure some diseases. One of banana species which is known to have a lot of nutrients is Tongka Langit banana (Musa troglodytarum), contains a much higher total carotene compared to the other, but no research has explored about the benefits of this banana. Several studies suggest that carotene can modulate innate and adaptive immune responses by activating macrophages, increasing or suppressing cytokine synthesis, and increasing the number and activation of lymphocytes which are very important to fight pathogens. In this study, fish growth and gene expression related to immune response were analyzed due to feeding mixed with Tongka Langit banana. Zebrafish (Danio rerio) is used as a model animal because it is a poliphagous fish. After 2 months of feeding, the food mixed with bananas was considered safe and well consumed because it showed a 100% survival rate and have a similar rate of growth as commercial feeding. The results of qRT-PCR analysis on marker genes related to the immune response in the intestine showed that the feed mixed with Tongka Langit banana was thought to be able to modulate the immune response through activation of macrophage and development of T helper type 2 cell, and was thought to has anti-inflammatory and immunoprotective activities because it was able to increase the relative expression of the IL-4, IL-1β, IL-8, and MHC Class II mRNA, and suppress the relative expression of TNF-⍺ and IL-6 mRNA genes. Therefore, Tongka Langit banana may can be used as a functional feed material which can later be used as the main reference for further functional food development.<p align="justify"> |
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