SECONDARY METABOLITES OF ENDOPHYTIC FUNGI Colletotrichum phyllanthi FROM FRUIT PETALS OF Physalis peruviana AND THEIR ANTIBACTERIAL ACTIVITY ALONG WITH EVALUATION OF EPIGENETIC MODIFIER PROCAINE HYDROCHLORIDE

• Main Author: AZIZAH - NIM: 20516016 , QIVI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30058
• Institution: Institut Teknologi Bandung

<p align="justify">Endophytic fungi are endosimbiotic organisms which live inside plant tissues without harming the host plant. Endophytic fungi produce some secondary metabolites with various important bioactivities. Physalis is known as one of medicinal plant that produces secondary metabolites with antibacterial activity. However, secondary metabolites evaluation of endophytic fungi from this plant has not been widely reported. Based on that fact, this study is aimed to isolate endophytic fungi from fruit petals of Physalis peruviana, to isolate its secondary metabolites, and to evaluate the effect of epigenetic modifier addition, prokain hidroklorida, into endophytic fungi media, and to antibacterials activity. Isolation of endophytic fungi was performed through sterillization, inoculation, and subcultur. Endophytic fungi was then cultivied in PDB (potato dextrose broth) in 14 days at 28o C with and without prokain hidroklorida addition. Furthermore, by extracting media with ethyl acetate and maceration micelia with methanol were obtained methanol extract and ethyl acetate extract 10 grams and 1,3 grams. Isolation of secondary metabolites from this extracts was performed through fractionation and purification with various techniques of chromatography. Antibacterial activities of extracts and pure compounds were performed by measuring MIC values against two gram-(+) bacteria and two gram-(-) bacteria. By this methodology, one endophytic fungi, Colletotrichum phyllanthi, and five pure compounds have been isolated. Structure elucidation of pure compounds has been done based on NMR 1D and 2D spectroscopy data. Two pure compounds have been isolated from methanol extract and identified as ergosterol (1) and ergosterol peroxide (2); nicotinic acid (3) from ethyl acetate extract; and from ethyl acetate extract with prokain hidroklorida addition, there were nectriapyrone (4) and tyrosol (5). There were some different compounds observed in TLC profile of polar fraction, after the addition of epigenetic modifier, procaine hydrochlorida. Antibacterial activity was also conducted with amoxicillin standard (MIC 1,56 – 6,25 &#956;g/mL). Extracts and pure compounds showed value of MIC > 500 &#956;g/mL and MIC >100 &#956;g/mL, respectively. These MIC values show that the isolated compunds and extracts were inactive. Moreover, it was also known that addition of epigenetic modifier, procaine hydrochloride, gives influence in secondary metabolites production.<p align="justify"> <br /> <br />