PREPARATION OF CHROMATOGRAPHY COLUMN BASED ON SILICA FUME MODIFIED BY AMINE FOR SUGAR SEPARATION: AN INITIAL STUDIES
The HPLC (High Performance Liquid Chromatography) separation method using silica columns <br /> <br /> modified by amine are widely used, especially in the separation of sugar mixture. In this research, <br /> <br /> silica fume column with particle size 325 - 400 mesh have b...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/30137 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The HPLC (High Performance Liquid Chromatography) separation method using silica columns <br />
<br />
modified by amine are widely used, especially in the separation of sugar mixture. In this research, <br />
<br />
silica fume column with particle size 325 - 400 mesh have been made. Silica fume was activated with <br />
<br />
standard piranha solution, then reacted with 3-APTES, whose diluted in methanol and demineralized <br />
<br />
water. FTIR characterization using DRS method showed the presence of a new peak at wavenumber <br />
<br />
1650 - 1500 cm-1. That peak is refer to amine functional group and can indicated that the <br />
<br />
modification was success. Silica fume modified by amine then tested for separation performance <br />
<br />
towards fructose and maltose mixture. Measurements were carried out by the FIA-UV method (Flow <br />
<br />
Injection Analysis-Ultraviolet) at wavelength 282 nm. Spectrum analysis showed the separation <br />
<br />
between fructose and maltose is happened. Silica fume after modified by amine also showed slimer <br />
<br />
peaks than silica fume before modified. |
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