#TITLE_ALTERNATIVE#

• Main Author: C A PANGSIBIDANG NIM : 10713029, REYNATHA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30358
• Institution: Institut Teknologi Bandung

Most currently available vaccines are delivered by intravenous injection. However, vaccine <br /> <br /> delivery through intravenous routes has several deficiencies such as invasive, infectious, costly <br /> <br /> production, and only effective to induce a systemic immune response because the antibodies <br /> <br /> barely reach the mucosa. Oral vaccines have several attractive features compared with <br /> <br /> parenteral vaccines. The challenge of oral delivery is a result of the obstacles presented by the <br /> <br /> GI tract. These obstacles include exposure to a wide range of pH environments, enzymatic <br /> <br /> degradation, and poor permeability across the intestinal epithelium. Therefore we used sodium <br /> <br /> alginate, Eudragit S100, and Eudragit FS 30D as enteric coating polymer. Furthermore, the <br /> <br /> greatest challenge in oral antigen formulation is the antigen reaches the specific target site, The <br /> <br /> specific site for delivery of the nanoparticles antigen targeting to the ileum region, i.e in the M <br /> <br /> cell in Follicle Associated Ephitelium (FAE) Peyer's patches (PPs). The dosage formulations are <br /> <br /> prepared in the form of nanoparticles to allow the preparation to be internalized by M cells to <br /> <br /> dendritic cells and macrophages. Phyllanthus niruri L. (PN) is used as an adjuvant to enhance <br /> <br /> immunogenicity of bovine serum albumin (BSA) nanoparticles. Nanoparticles are made by <br /> <br /> ionotropic gelation method using chitosan polymer with sodium tripolyphosphate as polymer <br /> <br /> crosslinker. Preparation of the nanoparticles was done using chitosan as a matrix to entrap BSA <br /> <br /> as an immunogen. PN dose used was 175; 350; and 700 &#956;g. The nanoparticles formed were <br /> <br /> characterized for particle size, polydispersity index, and entrapment efficiency. The entrapment <br /> <br /> efficiency of BSA and PN was determined using Bradford method and UV spectrophotometry <br /> <br /> with quercetin as a marker. The results of in vitro evaluation it is known that the most optimal <br /> <br /> polymer to protect NP-BPN nanoparticles is Eudragit FS 30D. While based on the results of in <br /> <br /> vivo evaluation on Day-9 after immunization showed that the best preparation formula that can <br /> <br /> enhance immune response was NP-E 700 &#956;g/ml. Based on In vivo evaluation NP-BPN 4 enhance <br /> <br /> the highest immune response. <br />