#TITLE_ALTERNATIVE#

• Main Author: AYU PRASTIWI NIM : 10714086, RIZKA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30565
• Institution: Institut Teknologi Bandung

Jojoba oil commonly used in cosmetic industry as emolient. &#946;-carotene is one type of carotenoids <br /> <br /> which contains antioxidant substances. &#946;-carotene is also contained in some vegetable oils such as <br /> <br /> palm oil and jojoba oil. The composition of jojoba oil is similar to human sebum’s composition which <br /> <br /> produced by sebaceous gland. The aim of this research is to develop a method in determining the <br /> <br /> concentration of &#946;-carotene present in jojoba oil (Simmondsia chinensis) by transesterification <br /> <br /> reaction using visible spectrophotometer method and determined the concentration of &#946;-carotene in <br /> <br /> jojoba oil. The method was developed to obtain the highest levels of &#946;-carotene based on the <br /> <br /> optimum condition in the preliminary reaction. Transesterification reaction used 1 grams of NaOH and <br /> <br /> 54 grams of metanol to produced 77,302 grams of the highest level fatty acid methyl ester (FAME). <br /> <br /> Validation method was performed to determine of linearity, limit of detection, limit of quantification <br /> <br /> ,accuracy, and precision. Based on the results of validation method, linearity test obtained r of 0.9995 <br /> <br /> and r <br /> <br /> 2 <br /> <br /> of 0.9992; accuracy test %recovery of 90.094 – 101.022 %; limit of detection is 0.89 &#956;g/mL and <br /> <br /> limit of quantification is 2.964 &#956;g/mL, precision test obtained relative standard deviation of 4.687; <br /> <br /> 1.246; 0.859 % for intraday test and 4.687 and 1.837% for interday test. Method is able to determine <br /> &#946;-carotene in jojoba oil and the level &#946;-carotene in jojoba oil is 155,641 &#956;g/mL. <br />