#TITLE_ALTERNATIVE#

• Main Author: PRAMADANI NIM : 10714069, RIZKY
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30623
• Institution: Institut Teknologi Bandung

Free radicals are compounds that have atoms with unpaired electrons which is very reactive. Antioxidants <br /> <br /> are electron donor compounds that can reduce the negative impact of radicals in the body, the example of <br /> <br /> antioxidant compounds are phenolic and flavonoid. Many plants contain phenolic and flavonoid compounds <br /> <br /> included macassar kernels. The objectives of this research were to study antioxidant activity of three organs <br /> <br /> of macassar kernels by determining IC50 of DPPH (2,2-diphenyl-1-picrylhydrazyl) and EC50 of CUPRAC (Cupric <br /> <br /> Ion Reducing Antioxidant Capacity); total phenolic and flavonoid content; analyze the correlation between <br /> <br /> total phenolic and flavonoid with their IC50 DPPH and EC50 CUPRAC, and analyze the correlation beetwen <br /> <br /> DPPH and CUPRAC in sample extracts. Quantitatives testing were performed by uv-visible <br /> <br /> spectrophotometry and correlations testing were analyzed by Pearson’s method. Ethyl acetate and ethanol <br /> <br /> extracts of unripe fruits, ripe fruits and leaves gave IC50 DPPH 0.41-33.79 µg/mL. The highest total phenolic <br /> <br /> content was given by the ethanolic leaves extract (14.66 ± 0.31 g GAE/100 g), while the ethyl acetate leaves <br /> <br /> extract showed the highest total flavonoid content (15.44 ± 0.27 g QE/100 g). Total phenolic content in <br /> <br /> unripe fruits, ripe fruits, and leaves extracts of macassar kernels had negative and significant correlation <br /> <br /> with their IC50 DPPH. IC50 DPPH of unripe fruits extracts had signicantly positive correlation with their EC50 <br /> <br /> CUPRAC. Ethyl acetate and ethanol extracts of unripe fruits, ripe fruits and leaves were categorized as very <br /> <br /> strong antioxidant by DPPH method. Phenolic compounds were the major contributors in antioxidant <br /> <br /> activity of unripe fruits, ripe fruits, and leaves extract of macassar kernels by DPPH method. DPPH and <br /> <br /> CUPRAC methods gave linear result in antioxidant activity of unripe fruits extracts. <br />