#TITLE_ALTERNATIVE#

• Main Author: KEVIN (10414010), SAMUEL
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30793
• Institution: Institut Teknologi Bandung

<p align="justify">Zika virus is one of the Flavivirus that recently caused epidemic in many country in South America in 2015-2016 and it has been associated with the cause of microcephaly in the babies in northeast Brazil. The serological method that become a main diagnostic method for Zika virus detection oftenly cannot generate a specific result, due to cross-reactivity between Zika virus amongst other Flavivirus. Therefore, the main goal of this research is to develop multi-epitope antigen that can differentiate infection caused by Zika virus from other Flavivirus by using Zika virus envelope protein as a target. In sillico analysis shows that 149SGMIVNDTGHETDENRAKVEITPNS PRAEATLGG182 and 273LEAEMDGAKGRLS285 are two amino acid sequence regions in the envelope protein that are Zika virus-specific B-cell epitope ; both of them are located in domain I and domain I/II, respectively. The PCR results that are electrophoresed using agarose gel shows band with the size of 882 bp, which is the amplicon size of the two predicted epitope sequences combined with linker protein L18 and tags protein. The DNA sequencing result shows 100% conservation rate, meaning it matches appropriately to the design of synthetic gene. The SDS-PAGE results which the protein size is around 30 kDa (different from the theoretical size, 24 kDa) shows that Zika virus multi-epitope antigen is successfully constructed in pET-32b(+) and estimatedly can be expressed in E.coli BL21 (DE3).<p align="justify">