MANGAN(II)-SALICYLIDENE-p-CHLOROANILINE COMPLEXES AS A SUPEROXIDE DISMUTASE MIMETIC ENZYME

• Main Author: DEWI UTAMANINGSIH NIM : 20513324, SENJA
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/30874
• Institution: Institut Teknologi Bandung

<p align="justify">Superoxide Dismutase (SOD) is a metalloenzyme which the active site contains a first transition metal ions (Cu2+, Zn2+, Mn2+, dan Fe2+). SOD is an enzyme that catalyzes the degradation reaction of superoxide anion (O2.-) to become hydrogen peroxide (H2O2) and oxygen (O2). The Schiff base metal complexes have been developed in order to achieve a potential SOD mimetic enzyme. In this research, the Mn(II) complex with salicylidene-p-chloroaniline has been synthesized which is formed from salicylaldehyde and p-chloroaniline precursors. Salicylidene-p-chloroaniline ligand was expected to form a bidentate ligand which contains one O donor atom and one N donor atom. The results of 1H NMR and 13C NMR spectroscopy shows that salicylidene-p-chloroaniline ligand has been formed. Salicylidene-p-chloroaniline ligand is used to synthesize Manganese(II)-salicylidene-p-chloroaniline complexes compounds. The UV-vis spectrum of Mn(II) complex showed absorbance with high intensity at 239 nm and 346 nm. Those absorbance assigned as a charge transfer transition. Based on the infrared spectrum, the binding of Mn(II) ion to those ligands was determined from the shifting of the wavenumbers at -OH and C=N of imine functional groups. The SOD activity was determined by non-enzyme assay (non-direct method) using the NBT/riboflavin reagent. This assay determined that the IC50 value of the Mn(II) complexes mixtures were found to be 0.89 &#956;&#924;. The cyclic voltammetry determination of complexes compound was perfomed in water as solvent of the analyte in the presence of KNO3 as the supporting electrolyte. Three electrodes used in this experiment were Au as working electrode, Pt as auxiliary electrode, and Ag/AgCl as reference electrode. The result showed that the E1/2 value of the Mn(II) complexes mixtures was 0.034 V, turn into a distance between -0,18 V - 0,91 V which is similiar to the E1/2 value of SOD in human body.