ISOLATION OF SECONDARY METABOLITE COMPOUNDS FROM HERB PHYSALIS MINIMA LINN

• Main Author: ULFAH NURHASANAH (NIM:20515037), SITI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31011
• Institution: Institut Teknologi Bandung

Ciplukan plants with another name cecenet or cecendet (Sunda), ceplukan (Java), <br /> <br /> nyurnyuran (Madura), and kopok-kopokan (Bali), is wild plants that ordinary <br /> <br /> grow in fertile soil, loose, and not inundated water. In gernerally people just think <br /> <br /> that ciplukan plants is weed plants (disturb other plants). Whereas this plant can <br /> <br /> used as woof and traditional medicine. Ciplukan include in genera Physalis that is <br /> <br /> family of Solanaceae, chemical content is compound that have structure C28- <br /> <br /> steroidal lakton. Ciplukan plants has 120 spesies that have bag close it’s friut and <br /> <br /> expands as the fruit grows. One of ciplukan species that researched is Physalis <br /> <br /> minima Linn. <br /> <br /> Dry herb powder Physalis minima Linn about 1,5 kg from UPT Materia Medica <br /> <br /> Batu – Malang. Isolation of secondary metabolite is extraction of herb Physalis <br /> <br /> minima Linn use methanol for 3 x 24 hours and replace the solvent every 24 <br /> <br /> hours. Separation and purification of secondary metabolite through some <br /> <br /> chromatography techniques, that is vacuum liquid chromatography, gravity <br /> <br /> column chromatography, and radial chromatography. Vacuum liquid <br /> <br /> chromatography done use silica gel Merck 60 GF254 as stationary phase and <br /> <br /> solvent n-heksana : ethyl asetate : methanol gradually as mobile phase so obtained <br /> <br /> 26 fraction that checked use TLC under UV rays &#955;254 and stain removal reagent. <br /> <br /> Gravity column chromatography continued from the fractination vacuum liquid <br /> <br /> chromatography using silica gel Merck 60 size 35-70 mesh as stationary phase <br /> <br /> and use solvent n-heksana : ethyl asetate as mobile phase. Radial chromatography <br /> <br /> done as purification of the results from gravity column chromatography. <br /> <br /> Caracterization of pure compounds of isolation conducted based on melting point <br /> <br /> data, FTIR, NMR 1D (1H-NMR use CDCL3 solvent which operate at frequencies <br /> <br /> 500 MHz and 13C-NMR use CDCL3 solvent which operate at frequencies 125 <br /> <br /> MHz) and NMR 2D (HSQC and HMBC). From herb extract of methanol Physalis <br /> <br /> minima Linn has successfully isolated the pure derivative compounds steroidal <br /> <br /> lactone, that are nicandrenone and nicandrenone-2 this compounds have never <br /> <br /> been found before in Physalis minima Linn (Solanaceae) as well as in other <br /> <br /> Physalis genera. Both of these compounds are found on Nicandra physalodes <br /> <br /> (Solanaceae) from Brazil.