#TITLE_ALTERNATIVE#

• Main Author: ULI MARBUN : 10714081, SONDANG
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31027
• Institution: Institut Teknologi Bandung

Magnetic particle (Fe3O4) is a particle that has characteristics such as superparamagnetic, easily separated <br /> <br /> with external magnets, and ability to be used for enzyme/protein immobilization. However, it is easily <br /> <br /> oxidized, thus a process is needed to increase particle stability, namely coating the particle with chitosan. <br /> <br /> In this research, Fe3O4 modified with chitosan (Fe3O4-C) was synthesized and its affinity with protein was <br /> <br /> observed. The protein used was peroxidase enzyme from radish. Synthesis of Fe3O4 dan Fe3O4-C was done <br /> <br /> using co-precipitation method. Chitosan was crosslinked on the surface of Fe3O4 with a combination of <br /> <br /> tripolyphosphate/sulfate. Characterization of both particles included particle weighing, magnetic <br /> <br /> properties, infrared spectrum, and their interaction with proteins. The results showed that the weights of <br /> <br /> Fe3O4 and Fe3O4-C were 1.20 ± 0.02 gram dan 0.11 ± 0.003 gram, respectively. Precipitation times of Fe3O4 <br /> <br /> and Fe3O4-C with and without magnets were different but both had magnetic properties. The results of <br /> <br /> interaction between particles and protein showed a decrease of the total protein amount and peroxidase <br /> <br /> activity. The largest decrease of total protein amount occurred at pH 7 for 30 minutes, which was 55% <br /> <br /> with peroxidase activity 0.01 U/mg. The particle resulted from the interaction between particles and <br /> <br /> protein solution (Fe3O4-P dan Fe3O4-CP) had peroxidase activity and magnetic properties, hence showing <br /> <br /> that Fe3O4 and Fe3O4-C can be used for enzyme immobilization. <br />