#TITLE_ALTERNATIVE#

• Main Author: FAUZIAH ALKAFF NIM:10513083, SYARIFAH
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31186
• Institution: Institut Teknologi Bandung

The tropical forests of Indonesia along with their biodiversity are the abundant natural resources for Indonesia. In Indonesia, there are about 30,000 species of plants and 7,500 of them are efficacious as medicines. However, only 1200-1400 species have been exploited by the community and 300 species of them have been used in the traditional medicines industries. One of the plants that can be developed as a source of medicines is mistletoe (Dendrophthoe pentandra). There are several of mistletoes that are usually used as traditional medicines, such as mango-mistletoe (the mistletoe that grows on mango trees) is commonly used as wound medicicnes, cough drugs, and nausea drugs. Previous research has reported that the extract of the clove-mistletoe has cytotoxic while mango-mistletoe has antiplasmodium activity. Then, the result of qualitative test on starfruit-mistletoe indicated that the extract contained flavonoids, triterpenoid, tannins, and saponins. In addition, one flavonoid compound, namely quercitrin, has been isolated as well. However, there are no phytochemistry of mango-mistletoe leaves and their anticancer activity. Therefore, the phytochemical study of mango-mistletoe leaves is very important to be conducted. In this research, the secondary metabolites have been isolated from mango-mistletoe collected from Cirebon, West Java. The secondary metabolites isolation included the extraction of mango-mistletoe powder using acetone solvent followed by the separation and purification of its secondary metabolites using vacuum liquid chromatography and gravitational column chromatography. Two compounds have been successfully isolated and their structures have been characterized based on spectroscopic data, i.e. NMR 1D (1H-NMR and 13C-NMR) and NMR 2D (HSQC and HMBC). Furthermore, the two compounds were suggested as flavonoid derivatives of the flavonol group, named as afzelin and quercitrin. Afzelin was for the first time isolated from mistletoe (Dendrophthoe pentandra), while quercitrin (5) has been found from the same species. Then, the extract and isolated compounds have been examined for their cytotoxic properties against murine leukemia P-388 cells using MTT method. This test is one of the preliminary assay for screening of anticancer potential compounds suggested by NCI (National Cancer Institute). The test resulted that the extract and isolated compounds were not active with IC50 values of 22.01 ?g /mL (acetone extract), 56.69 ?g/mL (quercitrin), and 49.79 ?g/mL (afzelin).