Reduction of Ammonium ( ) and Orthophosphate ( ) Content in Dairy Manure Wastewater Using Chlorella vulgaris with Variations in Frequency of Inoculum Addition

• Main Author: Adhitia Suyadi ( Nim :11214017), Teguh
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31255
• Institution: Institut Teknologi Bandung

In biogas production using dairy manure, an anaerobic digestion process also produced a by product bioslurry, also known as Anaerobically Digested Dairy Manure Wastewater (ADDMW), which is rich in nutrient. The availability of some organic compunds such as ammonium, phosphate, and other nutrients make ADDMW potentially to be used as growth media to grow autotrophic organism, including microalgae. Furthermore microalgae potentially could reduce organic content in ADDMW, thus play a role as phycoremediator to organic waste water. In this study we used ADDMW medium for the growth of microalgae, determining its growth kinetics and level of reduction in ammonium and orthophosphate content in ADDMW. In addition, we also analysed protein content in microalgae biomass for potential use as animal feed. The experiment was conducted using some variations in photoperiodism i.e. 16:8; 12:12; and 8:16 and medium dilution factor of 2.5 and 5 times. Furthermore, frequency of inoculum addition was also examined i.e. a) 80 mL on day 0 (once); b) 26.67 mL on day 0 to 2 (first 3 days); and c) 8 mL on days 0 to 9 (every day). The results showed that 16:8 photoperiod and 5 times medium dilution produced the highest biomass growth and productivity kinetics. Variation in frequency of inoculum addition showed no significant effect to a decrease in ammonium levels, but indicated some effect to orthophosphate level. Protein content in microalgae biomass cultivated in ADDMW showed slightly higher level (11.49 ± 0.51%) compared to protein content from biomass cultivated on synthetic medium (Bold Basal Medium) (9.29 ± 0, 23%).