Bioconversion Study of Dodecanedioic Acid from Fatty Acid and Its Derivatives using Candida tropicalis

• Main Author: ARIF SETIO NUGROHO (NIM : 13014002) - JATI PUTRA MAHARAJA (NIM : 13014069), THE
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31280
• Institution: Institut Teknologi Bandung

<p align="justify"> Dodecandioic acid (3DA) is heavily utilised in industry especially as the raw material for <br /> <br /> nylon 6,12. Nowadays, 3DA production focused on fermentation technology ustilising Candida tropicalis as the biocatalyst. This method appears to be advantageous due to its ability to utilise biomass derivatives such as lauric acid and methyl laurate as the substrate. In this study, the effect of fermentation substrate and concentration on cell growth and 3DA production by wild type Candida tropicalis isolated from Ambarella fruit shells in Indonesia was studied. The study was performed on lab scale flask with working volume of 300 mL. The type of substrate (lauric acid and methyl laurate) and <br /> <br /> substrate concentration (1, 3, and 5 g/L) were varied. To increase the substrate solubility, Tween 80 were added at a constant concentration of 1.67% v/v. The addition of surfactant could increase the solubility of lauric acid and methyl laurate by 13% and 91%, respectively. The substrates were utilised by Candida tropicalis for its growth, whereby the maximum biomass concentration was attained in methyl laurate system on the last day of experiment after substrate addition. The cell growth was followed by 3DA production in which the maximum yield was on the forth day after substrate addition. The maximum 3DA yield which is 0,247 g-3DA/ g-dissolved substrate was obtained with lauric acid while the maximum 3DA yield for methyl laurate is 0,144 g-3DA/ g-dissolved substrate. This research showed potential for 3DA synthesis by fully using renewable resources available naturally instead of using non-renewable petrochemical resources. <p align="justify">