Separation of Antimicrobial Components from Curcuma xanthorrhiza by Vacuum Liquid Chromatography Using Calcium Oxide (CaO) Column

• Main Author: FELIX DARPIETO ABIK (NIM: 10512060), THEODORUS
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31282
• Institution: Institut Teknologi Bandung

<p align="justify">Temulawak (Curcuma xanthorrhiza) is a well-known Indonesian medicinal plant. It has been reported that temulawak possesses an antimicrobial terpenoid compound named xanthorrhizol. In order to be applicable for industrial purposes, it is necessary to obtain xanthorrhizol-enriched fractions. Enrichment has been conducted in this research by vacuum liquid chromatography (VLC) using calcium oxide (CaO) as stationary phase. Fractionation on crude extract to stationary phase mass ratio between 1:60 to 1:20 produced identical fractionation pattern as confirmed by thin layer chromatography (TLC) analysis using n-hexane to EtOAc ratio of 9:1 and 1:1 v/v eluent system. TLC chromatogram shows a spot accounted for xanthorrhizol at Rf = 0.57 by comparison to the analysis of standard compound. 1H NMR spectrum analysis shows characteristic signals of xanthorrhizol. Fractionation method optimized using 1:20 crude extract to CaO mass ratio yields optimal elution scheme over eluent combination of n-hexane:EtOAc 10:0 (3 fractions of 35, 15, and 20 mL respectively), 4:1 (25 mL), 2:3 (25 mL), 1:4 (25 mL), and 0:10 (25 mL). Xanthorrhizol-enrichment is shown to be present within 2nd to 5th fractions. Quantitative analysis on the relative concentration of xanthorrhizol for the first 5 fractions using gas chromatography shows 10.23; 50.88; 73.68; 80.82; and 75.15% respectively. It has been concluded that an optimal fractionation method to achieve xanthorrhizol-enriched fraction has been obtained.<p align="justify">