#TITLE_ALTERNATIVE#

• Main Author: HANDOKO NIM : 11614019, YOGIE
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31747
• Institution: Institut Teknologi Bandung

Silymarin is natural active compounds isolated from Silybum marianum plant. Traditionally, <br /> <br /> silymarin has been used as a herbal medicine for the treatment of liver-related disorders. <br /> <br /> However, silymarin has a low bioavailability due to its poor absorption and rapid metabolism. This <br /> <br /> study was performed to develop nanoemulsion system containing silymarin to improve its <br /> <br /> bioavailability. The formulation was refered to the previous work. The oil phase of nanoemulsion <br /> <br /> consisted of castor oil, chremophor RH 40 as surfactant, and polyethylene glycol 400 as cosurfactant with a ratio of 1:8:1. The nanoemulsion was formed spontaneously by the addition of <br /> <br /> phosphat buffer at pH 6.4 under mild stirring. Characterization of nanoemulsion included visual <br /> <br /> appearance, droplet size, polydispersity index, zeta potential, entrapment efficiency, and physicalchemical stability under normal storage condition for 2 weeks. In addition, the characterization <br /> <br /> was also conducted in vitro using MCF-7 and MDA-MB cell lines to study the toxicity and cellular <br /> <br /> uptake capability. The toxicity assay to determine the IC50 value was done using MTS method [3- <br /> <br /> (4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]. <br /> <br /> Determination of IC50 was done using microplate reader on wavelength of 490 nm. Further, the <br /> <br /> interaction of sylimarin nanoemulsion and the solution on to MCF-7 cell line was analysed with <br /> <br /> confocal microscopy, after 3 and 6 hours incubation with concentration below IC50 value. When <br /> <br /> the confocal microscope was used to study the cellular uptake of the nanoemulsion, Carbon dot <br /> <br /> was applied and co-encapsulated with silymarin. Silymarin nanoemulsion has droplet size of 27.95 <br /> <br /> ± 4.31 nm, PI 0.296 ± 0.09 and charge of <br /> <br /> -11.81mV; and the entrapment efficiency was 91.92 ± 0.04 %. Nanoemulsion containing silymarin <br /> <br /> and carbon quantum dots showed good stabilty under studied condition. Based on the stability <br /> <br /> data, the nanoemulsion showed no significant changes of the entrapment efficiency as well as the <br /> <br /> physical characteristic. The IC50 value of silymarin nanoemulsion and solution was respectively <br /> <br /> 30.67 <br /> <br /> µg/mL and 45.40 µg/mL for MCF-7, 37.50 µg/mL and 39.14 µg/mL for MDA-MB. The <br /> <br /> nanoemulsion containing sylimarin is potential to penetrate into the MCF-7 cell line at <br /> <br /> concentration of 12.5 µg/mL both after 3 and 6 hours incubation. According to this study, the <br /> <br /> developed nanoemulsion is promising system to improve the therapeutic value of sylimarin. To <br /> <br /> confirm this conclusion, it is required further studies. <br />