Application of Marasmiellus palmivorus Laccase Enzyme Crude Extract in Oil Contaminated Soil for Hydrocarbon Biorecovery

• Main Author: C.K.A.S.W. - Nim: 10414003 , Yollanda
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31763
• Institution: Institut Teknologi Bandung

<p align="justify">Oil mining industry activities in Indonesia have caused many cases of oil pollution on the land. Based on Minister of Environment Regulation No. 2 of 2008 one of the activities to utilize petroleum waste is by recovering oil that has polluted the soil. The lacase enzyme is known to have the ability to catalyze the oxidation reaction of hydrocarbons, so it can be used as a biorecovery agent from contaminated soil. The purpose of this study is to determine the ability of lacase to be applied as an agent that can increase oil recovery from contaminated soil. Lakase is extracted from Marasmiellus palmivorus produced by solid state fermentation (SSF) method. The crude extract of lacase was tested by spectrophotometry using ABTS as. While protein concentration were tested by the Bradford Method. The land used is land from the Petroleum Mining Industrial Zone in Wonocolo Village, Bojonegoro Regency, East Java with concentration of TPH (Total Petrolrum Hydrocarbon) of 3.84% and 16.72%. Contaminated soil was incubated for 0, 6, 12, 18, 24, 30 and 36 hours with three variations of specific lacase activity (4.93; 10.39; 22.31 U / mg) as well, the control incubated with the addition of distilled water. Furthermore, measured concentration of TPH recovered and those remaining on the ground. In addition to knowing the SARA fraction (Saturated, Aromatic, Resin, and Asphalt) from the recovered oil, chromatography method was used with a stationary phase in the form of silica gel 60 - 200 mesh. The results showed that the specific activity of lacase 10.39 U / mg had the highest oil recovery ability in soil with TPH of 16.72% and 3.84% with the best incubation time of 12 hours for soil with TPH concentration of 16.72%. Whereas the soil with TPH concentration of 3.84% can be recovered optimally in incubation for 30 hours. The recovered fraction of soil with TPH concentration was 3.84% and 16.72% dominated by aromatic fractions with concentrations of 94.23% and 86.97% respectively. Therefore, Lakase Enzyme can be applied as a petroleum biorecovery agent in contaminated soil.<p align="justify">