#TITLE_ALTERNATIVE#

• Main Author: Dwi Putri - Nim: 10514024 , Yulia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/31812
• Institution: Institut Teknologi Bandung

<p align="justify">Tea has a high content of catechins (natural antioxidants). Catechin in tea consists of 4 major groups such as epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG. EGCG compounds act as natural antioxidants and they are mostly found in white tea. EGCG content in white tea can reach three times or more than other types of tea. EGCG in white tea can combat germs effectively so it is potential to be used as an antibacterial agent. The purpose of this study was to isolate epigallocatechin errors (EGCG) from white tea and study its activity as antibacterial to Gram positive bacteria, Baccillus cereus as well as againts Gram negative bacteria Escherichia coli. The method used was liquid-liquid extraction with 5 × 200 mL ethylacetate solvent and continued isolation by liquid-liquid extraction method with n-hexane solvent: acetone = 9: 1 (v / v) of 10 x 100 mL. The extract was analyzed using Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR). The antibacterial activity was tested using Kirby Bauer method. Extraction of 10.5269 g of white tea gave 1,6991 g (16,14%) crude extract and from 0,5081 g of crude extract gave EGCG 0,1099 g (21,62%), and caffeine 0.0991g (19.50%). HPLC chromatogram shows two types of catechins, they are ECG and EGCG as the largest component. Bioactvity test on E.coli using 100 mg / mL, 80 mg / mL, 60 mg / mL, and 40 mg / mL EGCG gave clear region of: 1.4 cm; 1.3 cm; 1.1 cm; and 0.9 cm. The antibacterial test with the same isolate concentration towards B.cereus gave a clear region of: 1,2 cm; 1.0 cm; 0.9 cm; and 0.8 cm. Bioactivity test using total plate count (TPC) towards E. coli resulted 538 colonies on medium without EGCG isolate and ampicillin, 1 colony on medium containing EGCG isolate 100 mg / ml and no colony on medium containing ampicillin 100 mg / ml . On the other hand, the same test againt towards B.cereus, gave 51 colonies on the medium without EGCG isolate and ampicillin, 25 colonies on medium containing EGCG isolate 100 mg / ml and 21 colonies on medium containing ampicillin 100 mg / ml. The bioactivity test proved that the EGCG isolate had a lower percentage of colonies lost than ampicillin and had higher toxicity levels against E.coli bacteria than B.cereus bacteria.<p align="justify">