TOXICITY TEST OF WATER-SOLUBLE FRACTIONS OF WASTE VEGETABLE OIL-BASED BIODIESEL AND BIODIESEL/DIESEL BLENDS ON Daphnia magna AND Allium cepa
Biodiesel mishandling, such as biodiesel spills and leakage to water has potential risks of environmental pollution. This research was aimed to determine the toxicity of water-soluble fractions of waste vegetable oil-based biodiesel and biodiesel/diesel blends. Acute toxicity testing was conducte...
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| Format: | Theses |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/32099 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Biodiesel mishandling, such as biodiesel spills and leakage to water has potential
risks of environmental pollution. This research was aimed to determine the
toxicity of water-soluble fractions of waste vegetable oil-based biodiesel and
biodiesel/diesel blends. Acute toxicity testing was conducted using Daphnia
magna and Allium cepa for genotoxicity testing. The analyses of Total Petroleum
Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) was performed
using Gas-Chromatograph Mass-Spectrophotometer (GCMS) method. Obtained
LC 50 value for water-soluble fractions of 0% biodiesel (B0), B2, B5, B10, B20,
B50, and B100 were 1.001 ppm; 1.279 ppm; 1.421 ppm; 1.577 ppm; 1.963 ppm;
3.026 ppm; and 3.157 ppm, respectively and chromosomal aberration percentage
of B0, B5, B20, and B100 were 35,60%; 26,71%; 25,33%; and 9,0%, respectively
at their EC 50. Results of correlation analysis between FAME either with LC 50
or percentage of chromosomal aberration showed a very strong correlation with
Pearson correlation coefficients were 0,906 and -0,975, respectively. This
research proved that there was a significant effect of F |
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