SYNTHESIS, CHARACTERIZATION, AND CELLULAR INTERACTIONS OF IRON OXIDE NANOPARTICLES AND GOLD-COATED IRON OXIDE NANOPARTICLES

• Main Author: Marvelous, Christian
• Format: Theses
• Language: Indonesia
• Subjects: Kimia anorganik
• Online Access: https://digilib.itb.ac.id/gdl/view/32168
• Institution: Institut Teknologi Bandung

Iron oxide nanoparticles have shown great promise as contrast agents for MRI. However, the use of iron oxide nanoparticles as contrast agent has shown some serious side effects, which resulted in the discontinuation of the production of already approved iron oxide nanoparticles. In order to overcome this problem another alternative started to emerge. Gold-coated iron oxide nanoparticles are more biocompatible, can be more easily functionalized, allowing to prolong the circulation time of these nanoparticles in blood. In order to investigate their biocompatibility, iron oxide nanoparticles and gold-coated iron oxide nanoparticles were synthesized and tested for their cellular uptake and toxicity. Iron oxide nanoparticles have been successfully synthesized using 2 different methods and confirmed by comparing the X-ray diffractograms with references. The X-ray diffractograms show similar peaks with reference, indicating the formation of iron oxide nanoparticles. A stable dispersion of iron oxide nanoparticles could be prepared in 25% TMAOH. The size of iron oxide nanoparticles is also measured by using Dynamic Light Scattering, Transmission Electron Microscope, and Scherrer’s equation using powder XRD. The particle size of stable iron oxide nanoparticles dispersion is found to be 10±2 nm and 10.10 nm as shown by TEM and Scherrer’s equation, respectively. DLS measurement shows the hydrodynamic size of 52 nm, and the dispersion remains stable for months. Gold-coated iron oxide nanoparticles have been synthesized using citrate reduction method. The presence of metallic gold is confirmed by the change of solution color to purple/burgundy. UV-visible spectrum of gold-coated iron oxide nanoparticles also shows the presence of gold, as indicated by the peak at 559 nm corresponds to LSPR (Localized Surface Plasmon Resonance) optical peak. The size of gold-coated iron oxide nanoparticles is measured by DLS and TEM method. The average particle size is 47.77 ± 8.40 nm as measured by TEM. TEM micrograph shows that gold-coated iron oxide nanoparticles have been successfully synthesized, and it suggested that aggregates of iron oxide nanoparticles are being coated instead of only single nanoparticle cores. iv Cellular uptake of iron oxide nanoparticles evaluated by Prussian blue staining method. Both HeLa cervical cancer cell and MCF-7 human breast cancer cell showing uptake of iron oxide nanoparticles at 50 ?g/mL. Both nanoparticles have been used to test its toxicity to HeLa and MCF-7 cell line. Iron oxide nanoparticles shows low or no toxicity up to the concentration of 250 ?g/mL for both cell lines. It was observed that MCF-7 is more prone to the toxicity of iron oxide nanoparticles, as shown by lower cell viability compared to HeLa cells. Gold-coated iron oxide nanoparticles have also low or no toxicity up to 250 ?g/mL. At the concentration of 500 ?g/mL, gold-coated iron oxide nanoparticles have shown at least the same toxicity with iron oxide nanoparticles. In MCF-7 cells, at the concentration of 500 ?g/mL, gold-coated iron oxide nanoparticles show very low toxicity contributed to high cell viability around 91%. These results indicate that gold-coated iron oxide nanoparticles might be more biocompatible than iron oxide nanoparticles. Future research of gold-coated iron oxide nanoparticles should focus on developing these particles further as they are very promising to be used in biomedical applications.