Biodiesel, Mass Spectrometry (MS), Liquified Petroleum Gas (LPG), Porphyridium cruentum, Scanning Electron Microscope (SEM).
The development of renewable energy sources have been a major focus of current research. Microalgae is one of the candidate because it contains lipids which can be transesterified to biodiesel. Generally, lipid extraction using hexane and chloroform is not economical. In the present study, the poten...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/32228 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The development of renewable energy sources have been a major focus of current research. Microalgae is one of the candidate because it contains lipids which can be transesterified to biodiesel. Generally, lipid extraction using hexane and chloroform is not economical. In the present study, the potential of liquified petroleum gas used to extract lipid of marine microalgae Porphyridium cruentum was investigated. P. cruentum cells were cultured in Walne medium for 8 d, and the biomass obtained was separated by the sentrifugation method. The lipid was extracted using liquified petroleum gas as a solvent in the home-made lipid extractor, then characterized by mass spectrometry (MS) and the residual of biomass were characterized by scanning electron microscope (SEM). The results showed that the biomass density of P. cruentum produced was 4.1 g/L with cell density of 1,1 x 107 cells/mL. Qualitative test of extracted lipid on oil absorbent paper resulted in transparent spots. SEM of residual biomass showed that cells which is sonicated released more lipids than non-sonicated cells. P. cruentum contained free fatty acid identified as EPA and AA, and triacyl glycerols as OOL, PlPlPl, EPA-PP and POO. |
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