Isolation and Antioxidant Activity Test of Biopigment from Marine Microalgae Thalassiosira sp. Cultivated on Modification Medium

• Main Author: Purwaningrum, Wulandari
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/32232
• Institution: Institut Teknologi Bandung

This research has been carried out in isolation of biopigment from marine microalgae Thalassiosira sp. and evaluation of their antioxidant activity by using 2,2-diphenyl-1- phycrylhydrazil (DPPH). The study was conducted through cultivation of microalgae in 3 variations of growth medium (Walne, NPK 10 %, modification medium), extraction of biopigments by using acetone p.a., purification of biopigments by using silica column chromatography, and characterization by using Thin Layer Chromatography (TLC)1 Dimensional and 2 Dimensional and visible spectrophotometry. The antioxidant activities against free radical DPPH were tested compare to ascorbic acid as a positive control. The result showed that the modification medium provide optimum biomass density of 3,66 g/L for 6 day cultivation. Five main visible biopigments from crude extract of acetone were spotted on TLC. Purified biopigments were identified as ?-carotene, chlorophyll a, chlorophyllide a, phaeophytin a, and diadinoxantin. The IC50 of ascorbic acid, phaeophytin a, ?-carotene, and diadinoxantin were 5,48 ; 29,01; 8,97 and 5,51 mg/mL respectively. The results suggested that ?-carotene and diadinoxantin from marine microalgae Thalassiosira sp. could be considered as potential source for antioxidant.