Bioactivity of Cu(II)-meso-tetraphenylsulfonate porphyrin and Zn(II)-meso-tetraphenylsulfonate porphyrin in DNA
Porphyrin derivative is one of photosensitizer group. In medical field, porphyrin derivative is commonly studied as antibacterial and anticancer drugs. Porphyrin structural modification is done to produce a porphyrin derivative that is soluble in water. In this study, structural modification of tetr...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/32257 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Porphyrin derivative is one of photosensitizer group. In medical field, porphyrin derivative is commonly studied as antibacterial and anticancer drugs. Porphyrin structural modification is done to produce a porphyrin derivative that is soluble in water. In this study, structural modification of tetraphenylporphyrin (TPP) was conducted by substituting sulfonate (-SO3H) on all TPP phenyl rings. Characterizations of spectroscopy UV-vis, fluorescence and mass spectrometry, shows that the structure of TPP was successfully modified and meso-tetraphenylsulfonate porphyrin (TPPS4) was produced. Metalloporphyrin of Zn (II) TPPS4 and Cu (II) TPPS4 was synthesized from mixture of TPPS4 solution and metal ion salt (Cu2+ or Zn2+), and pH of the solution was adjusted by using acetate buffer pH 4.8. Formation of both complexes was monitored by measuring the UV-vis spectroscopy and fluorescence. Bioactivity of the TPPS4 solution and metal ions mixture, with stoichiometric ratio 1:5, was studied in plasmid pGEM-T. DNA study shows that solution of the complex compound Zn (II) TPPS4, can cleave the DNA. |
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