NANOPARTICLE FORMULATION OF GENTAMISIN IN CHITOSAN-ACEMANNAN CONJUGATE

• Main Author: Saputra Yasir, Angga
• Format: Theses
• Language: Indonesia
• Subjects: Farmakologi dan terapeutik
• Online Access: https://digilib.itb.ac.id/gdl/view/32356
• Institution: Institut Teknologi Bandung

Staphylococcus aureus (SA) can cause both extracellular and intracellular infections. Gentamicin is an active antibiotic against SA extracellular infection, but its activity is poor against intracellular infections. Chitosan has been used as a carrier in intracellular drug delivery systems. This research aims to formulate gentamicin to resemble SA surface properties. Chitosan conjugate (Chi-Ace) is performed to improve the efficiency and efficacy of gentamicin. The conjugate reactions were carried out by dissolving chitosan with acemannan in acetic acid and then adding Sodium Borohydride (NaBH4) to reduce the chitosan amine bond on the acetyloxy group in acemannan. The production of Chi-Ace nanoparticles was performed by ionotropic gelation method using sodium tripolyphosphate (STPP). The acid acetate solution of Chi-Ace conjugate is mixed with gentamicin solution then crosslink with STPP. The optimization of the formula was performed using a response surface methodology consisting of ¼ factorial to identify significant factors and continued optimization with Box-Behnken design. The prediction of optimum formula consisted of: 10 mg of chitosan; STPP: Chitosan (0.28); 4.1 mg of gentamicin; sonication time 25 seconds and incubation time 30 minutes. The result of the confirmation of the formula shows the particle diameter, the efficiency entrapment and drug loading respectively 320 ± 22 nm; 51 ± 5%; 11.3 ± 0.88% . The release rate of gentamicin from the nanoparticle system at pH 5.2 is higher than at pH 7.4. Gentamicin solution and encapsulated gentamicin in nanoparticles is equal in minimum inhibitory concentration (16 ppm) and minimum bactericidal concentration (32 ppm).