ISOLATION AND CHARACTERIZATION OF PHYTASE FROM CHICKEN MANURE BACTERIA
Cereals, the main ingredient of animal feed, contain anti-nutrients of phytic acid (inositol phosphate) that capable of chelating proteins and cations. Phytase can be employed to reduce phytic acid in animal feed through the hydrolyzing phytic acid into free phosphate group and lower derivate of ino...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/32802 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Cereals, the main ingredient of animal feed, contain anti-nutrients of phytic acid (inositol phosphate) that capable of chelating proteins and cations. Phytase can be employed to reduce phytic acid in animal feed through the hydrolyzing phytic acid into free phosphate group and lower derivate of inositol phosphate. The aim of the present study was to isolate and characterize phytase obtained from chicken manure bacteria. The study included the isolation and screening of phytase-producing bacteria from chicken manure, the homology analysis of bacterium, and the determination of phytase activity. Phytase activity was measured from concentration of free phosphate. The results showed that one phytase-producing isolate obtained from poultry feces grew in the medium containing 5% rice bran extract at 37 °C for 5 d. Based on a phylogeny tree analysis of the related 16S rRNA genes, the isolate was identified as Acinetobacter sp. TZ1. The extracellular phytase expressed by Acinetobacter sp. TZ1 exhibited optimum reactions at pH 5 and 50 °C. The enzyme showed the activity of 64,6 nmol mL-1min-1 and specific activity of 236 nmol min-1mg-1. Relative molecular mass of phytase TZ1 predicted by SDS-PAGE was ~35 kDa. Phytase obtained is potential to improve animal feed quality by hydrolyzing phytic acid. |
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