DEHALOGENATION OF MONOCHLOROACETIC ACID BY HALOMONAS ELONGATA AG18

• Main Author: Shandriasti, Aughya
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/33664
• Institution: Institut Teknologi Bandung

Organohalogen is a group of compounds that widely applied in various industries, both as a solvent or as a mixture of compounds produced by the industries. Some industries that use organohalogen compounds include paint industries, pesticide industries and wide range of pharmaceutical industries. However, organohalogen compounds are difficult to be degraded in the environment, hence causing seriuos environmental pollution. Some bacteria have been identified to be able to degrade organohalogen compounds. Enzymes that catalyze the degradation of these organohalogen compounds known as dehalogenase. Halomonas elongata AG18 isolated from Bledug Kuwu mud crater, Central Java, is known as a bacterium that able to grow in a medium containing high levels of halogen. This research aims to study the ability of Halomonas elongata AG18 to degrade monochloroacetic acid, by growing the bacterium in a variety of media containing monochloroacetic acid. The activity of dehalogenase was studied by determining the concentration of chloride ion released into the medium employing colorimetric method of Bergmann and Sanik. The results showed that the dehalogenation activity by Halomonas elongata AG18 can occur in Luria Bertani medium with a minimum NaCl concentration of 0.8 mM and the maximum MCA concentration was 7 mM. The maximum chloride ions that are released by bacteria Halomonas elongata AG18 as a result of dehalogenation activity in Luria Bertani medium with the addition of 1 mM NaCl is 1.357 mM from initial substrate concentration of 7 mM with dehalogenation percentation reached 19,39%.