Fabrication of Electroactive Scaffold Based on Polycaprolactone- Polyaniline using 3D Printing Method for Bone Implant Application

• Main Author: Qulub, Fitriyatul
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/33950
• Institution: Institut Teknologi Bandung

Bone in skeletal structure is one of the human's vital tissues, to support human body and to protect soft inner organs. The damage of these bone tissue s such as fracture and osteoporosis will lead to the decrease of health‘s quality. Electroactive scaffold is one of the most recent and promising smart biomaterials for the treatment of bone tissues damage. This type of scaffold is interesting because its ability to increase the rate of bone cells-tissues regeneration. Furthermore, a well and precisely designed electroactive scaffold will provide an excellent environment for cell adhesion, proliferation and differentiation processes. In this sudy, research was conducted on electroactive scaffold based on polycaprolactone (PCL) as a matrix and polyaniline (PANI) as conductive filler with variation concentration of PANI (0.0, 0.1, 1.0, 2.0 wt.%) in the scaffold. The aims of this study are: (i) to synthesize Polyaniline emeraldine salt by chemical oxidation method directly as electroactive filler; (ii) to fabricate electroactive scaffold based on Polycaprolactone-Polyaniline (PCL-PANI) using 3D printing method; (iii) to characterize the influence of PANI concentration to the mechanical, electrical and biological properties of the scaffold. Fourier Transform Infra Red (FTIR) spectrum confirmed the formation of polyaniline emeraldine salt (PANI-ES) at peaks 1301 and 1138 cm-1. X-Ray Diffraction (XRD) analysis showed that degree of crytalinity reached 80 %. Scanning Electron Microscopy (SEM) images showed the produced PCL-PANI Scaffolds have homogenous and precises morphology with average pore dimension of 301.71 ± 9.22 ?m. The presence of PANI fillers in the PCL matrix can significantly increase compressive strength, cell viability and electrical conductivity. Scaffold with 0.1 % wt PANI concentration is the most effective as bone scaffold, because it has a compressive strength of 6.62 ± 0.59 MPa, increased human adipose-derived stem cell (hADSC) cell proliferation up to 14 days incubation by 20% against pure PCL based scaffold and electrical conductivity of 2.46 ± 0.85 x 10-4 S / cm. Based on the characteristic results in this study, electroactive scaffold based on PCL-PANI is a potential candidate for bone scaffold.