ISOLATION AND CHARACTERIZATION SOIL BACTERIA OF DEGRADING MONOCHLOROACETIC ACID (MCA)
Organohalogen is one of the biggest pollutants in the environment. These compounds are widely used as pesticides, herbicides, fungicide, and as an intermediate in the fertilizer industry, pharmaceuticals, and cosmetics. Bioremediation is one alternative step that can be done to reduce organohalogen...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/34139 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Organohalogen is one of the biggest pollutants in the environment. These compounds are widely used as pesticides, herbicides, fungicide, and as an intermediate in the fertilizer industry, pharmaceuticals, and cosmetics. Bioremediation is one alternative step that can be done to reduce organohalogen. Some soil bacteria are known to produce dehalogenases enzymes and thus potential to be used as bioremediator. This study aims to isolate soil bacteria that able to degrade monochloroacetic acid (MCA) and characterize their ability to grow in various concentrations of MCA. Isolation from soil samples from several areas produced 5 colonies of bacteria that able to grow on 10 mM MCA. Bacteria PG2 , PG3 , PW2 , and CW1 are Gram-negative while TJ4 are Gram-positive. PG2 and PG3 each able to release chloride ions by 88.81% and 95.14% in the medium containing 1 mM MCA, 26.16 % and 29.24% in 5mm MCA, and 8.72 % and 13.03% in 10 mM MCA. Meanwhile, TJ4 able to release chloride ions by
91.89% in 1 mM MCA, 28.17 % at 5mM MCA, and 12,09% in 10 mM MCA. On the other hand, PW2 and CW1 able to release chloride ions by 89.46% and
81.68% in 1 mM MCA, 28.10% and 24.31% in 5mM MCA, and 9.95% and
8.35% in 10 mM MCA. It could be seen that degradation occurs more effectively in the medium with low concentrations of MCA , in which PG3 show highest capability. All isolated bacteria reached stationary grow rate at 18-24 hours with OD600 of 0.3-0.4 .
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