(THE METHOD DEVELOPMENT FOR EXTRACTING LIPID FROM MARINE MICROALGAE NAVICULA SP. USING LIQUEFIED PETROLEUM GAS)

• Main Author: Indahsaputri, Awalia
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/34465
• Institution: Institut Teknologi Bandung

Microalgal lipid has been recognized as a promising alternative for biodiesel. Generally, lipid extraction from microalgae biomass by the use of organic solvents such as n-hexane and methanol: chloroform (1:1) is expensive. So, the development of efficient extraction methods is essential. The present study aims to develop method to extract lipid from the marine microalgae Navicula sp. biomass using Liquefied Petroleum Gas (LPG) in the home- made extractor. The research steps were Navicula sp. cultivation in modification medium, lipid extraction of the biomass (with and without sonication), qualitative examination of lipid, lipid characterization using Mass Spectrometry (MS), and biomass residue characterization using Scanning Electron Microscopy (SEM). The average of cell density and biomass density obtained at the 7 d culture was (2.93 ± 1.35) × 106 cell/mL and 1.02 ± 0.80 g/L. Positive results on oil absorbent paper suggested that algal lipid was successfully extracted from biomass, both with and without sonication of the cell. The cell morphology showed that the damage of biosilica cell wall due to the sonication treatment was observed. In addition, the MS peaks predicted that the tropical marine microalgae Navicula sp. lipid contained triacylglycerols of OOLa, POO, and OOLn as well as fatty acids of palmitoleic acid, palmitic acid, linoleic acid, oleic acid, and eicosapentaenoic acid (EPA).