ISSOLATION AND CHARACTERIZATION OF SECONDARY METHABOLITES FROM EUPHORBIA LEAVES (EUPHORBIA MILII)

• Main Author: Riyani, Ririn
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/34643
• Institution: Institut Teknologi Bandung

Euphorbia milii (Euphorbiaceae) is a nature to Madagascar and widely distributed in Indonesia. Euphorbia milii, known as ”Euphorbia” is used widely in the gardent as ornament. Several classes of coumpound have been described for E.milii, including, pentacyclic triterpenoids (? Amyrin acetate, and ? Amyrin acetate) ingenol derivates ( miliamines) and achetopenone derivates (2,4 dihydroxy-6-metoxyacetophenone). These compounds which have been isolated mostly from roots, stems, latex, and aerial parts of E.milii. The information about secondary metabolites from E.milii leaves is limited. This study aims to perform the isolation and characterization of secondary metabolites from E.milii leaves. Isolation of compounds is done through several process: extraction, fractionation, and purification. Pure compound which was obtained was characterized physical properties such as melting point and refractive index. The elucidation of the pure counponds was carried out by use of Ultra Violet, 1H- NMR, 13C-NMR, MS spectroscopy. Two compounds were isolated from the leaves of E. milii. The first compound (C-1) isolated from non-polar fractions (n- hexane: EtOAc = 8: 2). This compound (C-1) as white chrystal, melting point 255-2600C, less polar and gave positive reaction with Lieberman-Burchard reagent (indicated as terpenoid). This coumpond was not fluorescent under UV light. UV-Vis spectrum of this did not show any absorption peak. This indicates that this compound( C-1) has no double bonds and aromatic rings. The use of spectroscopic technique (1H-NMR, 13 C-NMR, DEPT, HMBC, and HMQC) indicated that this compound (C-1) is a pentacyclic triterpene alcohol with molecular formula C30H52O with the name A-Friedo-lupan-3ol. The second compound ( D-3) obtained from hexane : EtOAc = 7 : 3 fraction. The crude extrac was separated by radial chromatography and sephadex column chromatography. These Isolation and purification give pure coumpound (D-3) of 11 mg, greenish yellow solid. Characterization is done by using UV-Vis spectra, 1H-NMR, and ESI-IT-MS. Based on the spectrum analysis we concluded that the suggested structure for D-3 is 2,4-dihydroxy-6-metoxyacetophenone. This compound (2,4-dihydroxy-6-metoxyacetophenone) is not a new compound, this compound has been isolated previously from Euphorbia milii Desmoul. Ex Boiss, Euphorbia fischeriana, Artemisia annua L, and Artemisia Arfa. To give more complete and meaningful information about these compound’s profile, the next study about toxicity and bioactivity are expected.