EFFECT OF FGF-2 AND BMP-2 ON RAT (Rattus norvegicus) BONE MARROW STEM CELLS DIFFERENTIATION

• Main Author: M Lauda Feroniasanti, Y
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/34929
• Institution: Institut Teknologi Bandung

Myocardial infarction and ischemic become the leading cause of heart disease. Blood vessel occlusion can causes ischemic, and this condition can disrupt the nutrition supply to myocardial, leading to myocardial infarction. Transplantation of stem cells become a potential therapy for this disease. Stem cells are pluripotent cells that have the ability to differentiate into specific type of cells. Stem cells that have been induced to differentiate can be transplanted in the infarct area and cause tissue regeneration. Cell differentiation into specific type of cells mostly induced by external factors, for example growth factors. In this research, Fibroblast Growth Factor-2 (FGF-2), Bone Morphogenetic Protein-2 (BMP-2) and SU5402 were used to induce the cell differentiation into cardiomyocyte and endothelial cells. Analysis of cell differentiation were done in mRNA level using Reverse Transcription – Polymerase Chain Reaction (RTPCR) and Real Time-PCR. RT-PCR was done for Cardiac Troponin-I (CTN-I) gene and Myocyte Enhancer Factor-2c (MEF-2c) gene as cardiomyocyte marker, and for Vascular Endothelial Growth Factor-A (VEGF-A) gene and VEGFreceptor (VEGFR) gene as endothelial cell marker. Real Time-PCR was done for VEGFR gene. Treatment was devided into two groups, those were group treated for 6 days and group treated for 8 days. Each group consisted of 3 treatment groups, namely K (control, no treatment), P1 (treated with FGF-2 and BMP-2), and P2 (treated with FGF-2, BMP-2, and SU5402). Stem cells differentiation was showed by the alteration of cell morphology into spindle shape. Total RNA were isolated at the end of treatment period, that was at day 6 and day 8. RT-PCR for CTN-I gene and MEF-2c gene showed that CTN-I and MEF-2c were not expressed in cells, this meant that cells did not differentiate into cardiomyocyte in all treatment. RT-PCR for VEGF-A and VEGFR gene showed that VEGF-A gene was expressed in all treatments, whereas VEGFR gene was expressed only in P1 and P2 treatments from 8 days treatment group. Real Time-PCR analysis showed that all samples expressed VEGFR gene. Alignment between primer sequences and VEGFR gene sequence showed that VEGFR gene that detected in RT-PCR was VEGFR-1, and VEGFR gene that detected in Real Time-PCR was VEGFR-3. This result indicated that rat bone marrow stem cells differentiated into endothelial cells. It showed that FGF-2, BMP-2, and SU5402 could induce the differentiation of rat bone marrow stem cells into endothelial cells.