ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS

Chlorophyll is a natural pigment that is usually found in many green plants. Because of its structure that have conjugated double bonds, the chlorophyll can be explored its potential as an optical material. In this research, chlorophyll was isolated from leaves of spinach (Amaranthus Spec div.). The...

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Main Author: Ditya Sandiningtyas, Rachma
Format: Final Project
Language:Indonesia
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Online Access:https://digilib.itb.ac.id/gdl/view/35307
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Institution: Institut Teknologi Bandung
Language: Indonesia
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spelling id-itb.:353072019-02-25T09:03:17ZISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS Ditya Sandiningtyas, Rachma Kimia Indonesia Final Project Fe-pheophytin a, chlorophyll a, decay lifetime, optical material INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/35307 Chlorophyll is a natural pigment that is usually found in many green plants. Because of its structure that have conjugated double bonds, the chlorophyll can be explored its potential as an optical material. In this research, chlorophyll was isolated from leaves of spinach (Amaranthus Spec div.). The separation of chlorophyll a was conducted by using the sucrose column chromatography technique. The pure chlorophyll a then further modified by replacing the central ion of chlorophyll a, which is Mg2 + became Fe2+ to form Fe-pheophytin a. The optical characteristic and purity of chlorophyll a, pheophytin a, and Fe-pheophytin a were carried out using spectroscopic methods. The different characteristics of chlorophyll a and Fe-pheophytin a were distinguished from each other by the examination of their absorption and emission spectra. From absorbance measurements, there was a shift in the absorption peak of Fe-pheophytin a, which compared with chlorophyll a. The maxima of absorption of Fe-pheophytin a were observed at 393 nm in Soret band and 627 nm in Q band, while in the case of chlorophyll a were at 413 nm and 668 nm, respectively. Based on fluorescence measurement, it was observed that Fe-pheophytin a gives a red shift relatives to chlorophyll at 702.5 nm. The photostability of chlorophyll a and Fe-pheophytin a were studied by comparing the decay lifetime based on the fluoresescence measurement. Here, the decay lifetime of Fe-pheophytin a was observed 43.88 seconds, which is much longer than 12.79 seconds of chlorophyll a. This result suggests that Fe-pheophytin a is more stable intrisically than chlorophyll a. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
topic Kimia
spellingShingle Kimia
Ditya Sandiningtyas, Rachma
ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS
description Chlorophyll is a natural pigment that is usually found in many green plants. Because of its structure that have conjugated double bonds, the chlorophyll can be explored its potential as an optical material. In this research, chlorophyll was isolated from leaves of spinach (Amaranthus Spec div.). The separation of chlorophyll a was conducted by using the sucrose column chromatography technique. The pure chlorophyll a then further modified by replacing the central ion of chlorophyll a, which is Mg2 + became Fe2+ to form Fe-pheophytin a. The optical characteristic and purity of chlorophyll a, pheophytin a, and Fe-pheophytin a were carried out using spectroscopic methods. The different characteristics of chlorophyll a and Fe-pheophytin a were distinguished from each other by the examination of their absorption and emission spectra. From absorbance measurements, there was a shift in the absorption peak of Fe-pheophytin a, which compared with chlorophyll a. The maxima of absorption of Fe-pheophytin a were observed at 393 nm in Soret band and 627 nm in Q band, while in the case of chlorophyll a were at 413 nm and 668 nm, respectively. Based on fluorescence measurement, it was observed that Fe-pheophytin a gives a red shift relatives to chlorophyll at 702.5 nm. The photostability of chlorophyll a and Fe-pheophytin a were studied by comparing the decay lifetime based on the fluoresescence measurement. Here, the decay lifetime of Fe-pheophytin a was observed 43.88 seconds, which is much longer than 12.79 seconds of chlorophyll a. This result suggests that Fe-pheophytin a is more stable intrisically than chlorophyll a.
format Final Project
author Ditya Sandiningtyas, Rachma
author_facet Ditya Sandiningtyas, Rachma
author_sort Ditya Sandiningtyas, Rachma
title ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS
title_short ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS
title_full ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS
title_fullStr ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS
title_full_unstemmed ISOLATION OF CHLOROPHYLL A FROM SPINACH (AMARANTHUS SPEC DIV.) AND ITS MODIFICATION USING FE2+ IN PHOTOSTABILITY STUDY AS OPTICAL MATERIALS
title_sort isolation of chlorophyll a from spinach (amaranthus spec div.) and its modification using fe2+ in photostability study as optical materials
url https://digilib.itb.ac.id/gdl/view/35307
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